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[[Image:1mme.jpg|left|200px]]


{{Structure
==THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE==
|PDB= 1mme |SIZE=350|CAPTION= <scene name='initialview01'>1mme</scene>, resolution 3.100&Aring;
<StructureSection load='1mme' size='340' side='right'caption='[[1mme]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
<table><tr><td colspan='2'>[[1mme]] is a 4 chain structure. The May 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Self-splicing RNA''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_5 10.2210/rcsb_pdb/mom_2005_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MME FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
|GENE=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mme OCA], [https://pdbe.org/1mme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mme RCSB], [https://www.ebi.ac.uk/pdbsum/1mme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mme ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mme OCA], [http://www.ebi.ac.uk/pdbsum/1mme PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mme RCSB]</span>
}}


'''THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE'''
==See Also==
 
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
We have solved the crystal structure of an all-RNA hammerhead ribozyme having a single 2'-O-methyl cytosine incorporated at the active site to prevent cleavage. The conditions used differ from those in another recent solution in four significant ways: first, it is an all-RNA ribozyme rather than a DNA-RNA hybrid; second, the connectivity of the ribozyme backbone strands is different; third, the crystals were grown in the presence of a much lower concentration of salt; and fourth, the crystal packing scheme is very different. Nevertheless, the three-dimensional structure of the all-RNA hammerhead ribozyme is similar to the previous structure. Five potential Mg(II)-binding sites are identified, including one positioned near the ribozyme catalytic pocket. Upon this basis, as well as upon comparisons with the metal-binding sites in the structurally homologous uridine turn of tRNAPhe, we propose a mechanism for RNA catalytic cleavage.
[[Category: Large Structures]]
 
[[Category: RCSB PDB Molecule of the Month]]
==About this Structure==
1MME is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. The following page contains interesting information on the relation of 1MME with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb65_1.html Self-splicing RNA]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA].
 
==Reference==
The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage., Scott WG, Finch JT, Klug A, Cell. 1995 Jun 30;81(7):991-1002. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7541315 7541315]
[[Category: Protein complex]]
[[Category: Self-splicing RNA]]
[[Category: Self-splicing RNA]]
[[Category: Finch, J T.]]
[[Category: Finch JT]]
[[Category: Klug, A.]]
[[Category: Klug A]]
[[Category: Scott, W G.]]
[[Category: Scott WG]]
[[Category: catalytic rna]]
[[Category: loop]]
[[Category: rna hammerhead ribozyme]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:18:03 2008''

Latest revision as of 10:45, 14 February 2024

THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGETHE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE

Structural highlights

1mme is a 4 chain structure. The May 2005 RCSB PDB Molecule of the Month feature on Self-splicing RNA by David S. Goodsell is 10.2210/rcsb_pdb/mom_2005_5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.1Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

1mme, resolution 3.10Å

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