4cw7: Difference between revisions
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==Structure of the Fap7-Rps14 complex in complex with ATP== | ==Structure of the Fap7-Rps14 complex in complex with ATP== | ||
<StructureSection load='4cw7' size='340' side='right' caption='[[4cw7]], [[Resolution|resolution]] 2.46Å' scene=''> | <StructureSection load='4cw7' size='340' side='right'caption='[[4cw7]], [[Resolution|resolution]] 2.46Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4cw7]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CW7 OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4cw7]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_abyssi_GE5 Pyrococcus abyssi GE5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CW7 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.46Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
< | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cw7 OCA], [https://pdbe.org/4cw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cw7 RCSB], [https://www.ebi.ac.uk/pdbsum/4cw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cw7 ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/KAD6_PYRAB KAD6_PYRAB] Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates (By similarity). | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Bareille | [[Category: Pyrococcus abyssi GE5]] | ||
[[Category: Blaud | [[Category: Bareille J]] | ||
[[Category: Chardon | [[Category: Blaud M]] | ||
[[Category: Charenton | [[Category: Chardon F]] | ||
[[Category: Delbos | [[Category: Charenton C]] | ||
[[Category: Deschamps | [[Category: Delbos L]] | ||
[[Category: Jombart | [[Category: Deschamps P]] | ||
[[Category: Lebaron | [[Category: Jombart J]] | ||
[[Category: Leulliot | [[Category: Lebaron S]] | ||
[[Category: | [[Category: Leulliot N]] | ||
[[Category: Rety | [[Category: Loc'h J]] | ||
[[Category: Robert-Paganin | [[Category: Rety S]] | ||
[[Category: Tollervey | [[Category: Robert-Paganin J]] | ||
[[Category: Zhang | [[Category: Tollervey D]] | ||
[[Category: Zhang E]] | |||
Latest revision as of 14:15, 9 May 2024
Structure of the Fap7-Rps14 complex in complex with ATPStructure of the Fap7-Rps14 complex in complex with ATP
Structural highlights
FunctionKAD6_PYRAB Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates (By similarity). Publication Abstract from PubMedDuring biogenesis of the 40S and 60S ribosomal subunits, the pre-40S particles are exported to the cytoplasm prior to final cleavage of the 20S pre-rRNA to mature 18S rRNA. Amongst the factors involved in this maturation step, Fap7 is unusual, as it both interacts with ribosomal protein Rps14 and harbors adenylate kinase activity, a function not usually associated with ribonucleoprotein assembly. Human hFap7 also regulates Cajal body assembly and cell cycle progression via the p53-MDM2 pathway. This work presents the functional and structural characterization of the Fap7-Rps14 complex. We report that Fap7 association blocks the RNA binding surface of Rps14 and, conversely, Rps14 binding inhibits adenylate kinase activity of Fap7. In addition, the affinity of Fap7 for Rps14 is higher with bound ADP, whereas ATP hydrolysis dissociates the complex. These results suggest that Fap7 chaperones Rps14 assembly into pre-40S particles via RNA mimicry in an ATP-dependent manner. Incorporation of Rps14 by Fap7 leads to a structural rearrangement of the platform domain necessary for the pre-rRNA to acquire a cleavage competent conformation. RNA mimicry by the fap7 adenylate kinase in ribosome biogenesis.,Loc'h J, Blaud M, Rety S, Lebaron S, Deschamps P, Bareille J, Jombart J, Robert-Paganin J, Delbos L, Chardon F, Zhang E, Charenton C, Tollervey D, Leulliot N PLoS Biol. 2014 May 13;12(5):e1001860. doi: 10.1371/journal.pbio.1001860., eCollection 2014 May. PMID:24823650[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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