1mi7: Difference between revisions

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-[[Image:1mi7.gif|left|200px]]
- {{Structure
+==Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol==
-|PDB= 1mi7 |SIZE=350|CAPTION= <scene name='initialview01'>1mi7</scene>, resolution 2.50&Aring;
+<StructureSection load='1mi7' size='340' side='right'caption='[[1mi7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>
+<table><tr><td colspan='2'>[[1mi7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MI7 FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-|GENE= trpR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi7 OCA], [https://pdbe.org/1mi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mi7 RCSB], [https://www.ebi.ac.uk/pdbsum/1mi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi7 ProSAT]</span></td></tr>
-|RELATEDENTRY=[[1wrp|1WRP]], [[2wrp|2WRP]], [[3wrp|3WRP]], [[1tro|1TRO]], [[1trr|1TRR]]
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi7 OCA], [http://www.ebi.ac.uk/pdbsum/1mi7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mi7 RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/TRPR_ECOLI TRPR_ECOLI] This protein is an aporepressor. When complexed with L-tryptophan it binds the operator region of the trp operon (5'-ACTAGT-'3') and prevents the initiation of transcription. The complex also regulates trp repressor biosynthesis by binding to its regulatory region.
+== Evolutionary Conservation ==
-'''Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol'''
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
-==Overview==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mi/1mi7_consurf.spt"</scriptWhenChecked>
-The E. coli trp repressor (trpR) homodimer recognizes its palindromic DNA binding site through a pair of flexible helix-turn-helix (HTH) motifs displayed on an intertwined helical core. Flexible N-terminal arms mediate association between dimers bound to tandem DNA sites. The 2.5 A X-ray structure of trpR crystallized in 30% (v/v) isopropanol reveals a substantial conformational rearrangement of HTH motifs and N-terminal arms, with the protein appearing in the unusual form of an ordered 3D domain-swapped supramolecular array. Small angle X-ray scattering measurements show that the self-association properties of trpR in solution are fundamentally altered by isopropanol.
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==About this Structure==
+  </jmolCheckbox>
-MI7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI7 OCA].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mi7 ConSurf].
+<div style="clear:both"></div>
-==Reference==
+__TOC__
-E. coli trp repressor forms a domain-swapped array in aqueous alcohol., Lawson CL, Benoff B, Berger T, Berman HM, Carey J, Structure. 2004 Jun;12(6):1099-108. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15274929 15274929]
+</StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Benoff, B.]]
+[[Category: Benoff B]]
-[[Category: Berger, T.]]
+[[Category: Berger T]]
-[[Category: Berman, H M.]]
+[[Category: Berman HM]]
-[[Category: Carey, J.]]
+[[Category: Carey J]]
-[[Category: Lawson, C L.]]
+[[Category: Lawson CL]]
-[[Category: alcohol induced conformational rearrangement]]
-[[Category: dna binding protein]]
-[[Category: domain swapping]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:16:21 2008''