4mz2: Difference between revisions
No edit summary |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Crystal structure of the voltage-gated sodium channel beta 4 subunit extracellular domain== | ==Crystal structure of the voltage-gated sodium channel beta 4 subunit extracellular domain== | ||
<StructureSection load='4mz2' size='340' side='right' caption='[[4mz2]], [[Resolution|resolution]] 1.72Å' scene=''> | <StructureSection load='4mz2' size='340' side='right'caption='[[4mz2]], [[Resolution|resolution]] 1.72Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4mz2]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4mz2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MZ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MZ2 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.722Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mz2 OCA], [https://pdbe.org/4mz2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mz2 RCSB], [https://www.ebi.ac.uk/pdbsum/4mz2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mz2 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Disease == | == Disease == | ||
[ | [https://www.uniprot.org/uniprot/SCN4B_HUMAN SCN4B_HUMAN] Romano-Ward syndrome. The disease is caused by mutations affecting the gene represented in this entry. | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/SCN4B_HUMAN SCN4B_HUMAN] Modulates channel gating kinetics. Causes negative shifts in the voltage dependence of activation of certain alpha sodium channels, but does not affect the voltage dependence of inactivation (By similarity). | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 23: | Line 22: | ||
==See Also== | ==See Also== | ||
*[[Ion channels|Ion channels]] | *[[Ion channels 3D structures|Ion channels 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Das S]] | ||
[[Category: | [[Category: Van Petegem F]] | ||
Latest revision as of 19:45, 20 September 2023
Crystal structure of the voltage-gated sodium channel beta 4 subunit extracellular domainCrystal structure of the voltage-gated sodium channel beta 4 subunit extracellular domain
Structural highlights
DiseaseSCN4B_HUMAN Romano-Ward syndrome. The disease is caused by mutations affecting the gene represented in this entry. FunctionSCN4B_HUMAN Modulates channel gating kinetics. Causes negative shifts in the voltage dependence of activation of certain alpha sodium channels, but does not affect the voltage dependence of inactivation (By similarity). Publication Abstract from PubMedVoltage-gated sodium (Nav) channels are embedded in a multicomponent membrane signaling complex that plays a crucial role in cellular excitability. Although the mechanism remains unclear, beta-subunits modify Nav channel function and cause debilitating disorders when mutated. While investigating whether beta-subunits also influence ligand interactions, we found that beta4 dramatically alters toxin binding to Nav1.2. To explore these observations further, we solved the crystal structure of the extracellular beta4 domain and identified (58)Cys as an exposed residue that, when mutated, eliminates the influence of beta4 on toxin pharmacology. Moreover, our results suggest the presence of a docking site that is maintained by a cysteine bridge buried within the hydrophobic core of beta4. Disrupting this bridge by introducing a beta1 mutation implicated in epilepsy repositions the (58)Cys-containing loop and disrupts beta4 modulation of Nav1.2. Overall, the principles emerging from this work (i) help explain tissue-dependent variations in Nav channel pharmacology; (ii) enable the mechanistic interpretation of beta-subunit-related disorders; and (iii) provide insights in designing molecules capable of correcting aberrant beta-subunit behavior. Crystallographic insights into sodium-channel modulation by the beta4 subunit.,Gilchrist J, Das S, Van Petegem F, Bosmans F Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):E5016-24. doi:, 10.1073/pnas.1314557110. Epub 2013 Dec 2. PMID:24297919[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||