3pg2: Difference between revisions
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==The Crystal structure of the major pilin GBS80 of Streptococcus agalactiae 35 kDa C-terminal fragment== | ==The Crystal structure of the major pilin GBS80 of Streptococcus agalactiae 35 kDa C-terminal fragment== | ||
<StructureSection load='3pg2' size='340' side='right' caption='[[3pg2]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='3pg2' size='340' side='right'caption='[[3pg2]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3pg2]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3pg2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_V Streptococcus agalactiae serogroup V]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PG2 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pg2 OCA], [https://pdbe.org/3pg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pg2 RCSB], [https://www.ebi.ac.uk/pdbsum/3pg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pg2 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8E0S9_STRA5 Q8E0S9_STRA5] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 3pg2" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 3pg2" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Pilin 3D structures|Pilin 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Streptococcus agalactiae serogroup V]] | ||
[[Category: | [[Category: Narayana SVL]] | ||
[[Category: | [[Category: Vengadesan K]] | ||
Latest revision as of 05:16, 21 November 2024
The Crystal structure of the major pilin GBS80 of Streptococcus agalactiae 35 kDa C-terminal fragmentThe Crystal structure of the major pilin GBS80 of Streptococcus agalactiae 35 kDa C-terminal fragment
Structural highlights
FunctionPublication Abstract from PubMedThe Gram-positive pathogen Streptococcus agalactiae, known as group B Streptococcus (GBS), is the leading cause of bacterial septicemia, pneumonia, and meningitis among neonates. GBS assembles two types of pili-pilus islands (PIs) 1 and 2-on its surface to adhere to host cells and to initiate colonization for pathogenesis. The GBS PI-1 pilus is made of one major pilin, GBS80, which forms the pilus shaft, and two secondary pilins, GBS104 and GBS52, which are incorporated into the pilus at various places. We report here the crystal structure of the 35-kDa C-terminal fragment from GBS80, which is composed of two IgG-like domains (N2-N3). The structure was solved by single-wavelength anomalous dispersion using sodium-iodide-soaked crystals and diffraction data collected at the home source. The N2 domain exhibits a cnaA/DEv-IgG fold with two calcium-binding sites, while the N3 domain displays a cnaB/IgG-rev fold. We have built a model for full-length GBS80 (N1, N2, and N3) with the help of available homologous major pilin structures, and we propose a model for the GBS PI-1 pilus shaft. The N2 and N3 domains are arranged in tandem along the pilus shaft, whereas the respective N1 domain is tilted by approximately 20 degrees away from the pilus axis. We have also identified a pilin-like motif in the minor pilin GBS52, which might aid its incorporation at the pilus base. A Model for Group B Streptococcus Pilus Type 1: The Structure of a 35-kDa C-Terminal Fragment of the Major Pilin GBS80.,Vengadesan K, Ma X, Dwivedi P, Ton-That H, Narayana SV J Mol Biol. 2011 Apr 15;407(5):731-43. Epub 2011 Feb 17. PMID:21333654[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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