4gof: Difference between revisions

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==Crystal structure of the SGTA homodimerization domain with covalent modifications to both C38==
==Crystal structure of the SGTA homodimerization domain with covalent modifications to both C38==
<StructureSection load='4gof' size='340' side='right' caption='[[4gof]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
<StructureSection load='4gof' size='340' side='right'caption='[[4gof]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4gof]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GOF FirstGlance]. <br>
<table><tr><td colspan='2'>[[4gof]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GOF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4goc|4goc]], [[4god|4god]], [[4goe|4goe]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gof OCA], [https://pdbe.org/4gof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gof RCSB], [https://www.ebi.ac.uk/pdbsum/4gof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gof ProSAT]</span></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SGT, SGT1, SGTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gof OCA], [http://pdbe.org/4gof PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gof RCSB], [http://www.ebi.ac.uk/pdbsum/4gof PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gof ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/SGTA_HUMAN SGTA_HUMAN]] Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.<ref>PMID:18759457</ref>
[https://www.uniprot.org/uniprot/SGTA_HUMAN SGTA_HUMAN] Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.<ref>PMID:18759457</ref>  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</div>
</div>
<div class="pdbe-citations 4gof" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 4gof" style="background-color:#fffaf0;"></div>
==See Also==
*[[Small glutamine-rich tetratricopeptide repeat containing protein alpha|Small glutamine-rich tetratricopeptide repeat containing protein alpha]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Chartron, J W]]
[[Category: Large Structures]]
[[Category: Clemons, W M]]
[[Category: Chartron JW]]
[[Category: VanderVelde, D G]]
[[Category: Clemons Jr WM]]
[[Category: Four-helix bundle]]
[[Category: VanderVelde DG]]
[[Category: Protein binding]]
[[Category: Protein-protein interaction]]
[[Category: Ubl4a ubiquitin-like domain]]

Latest revision as of 10:07, 26 October 2022

Crystal structure of the SGTA homodimerization domain with covalent modifications to both C38Crystal structure of the SGTA homodimerization domain with covalent modifications to both C38

Structural highlights

4gof is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SGTA_HUMAN Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.[1]

Publication Abstract from PubMed

In the cytoplasm, the correct delivery of membrane proteins is an essential and highly regulated process. The posttranslational targeting of the important tail-anchor membrane (TA) proteins has recently been under intense investigation. A specialized pathway, called the guided entry of TA proteins (GET) pathway in yeast and the transmembrane domain recognition complex (TRC) pathway in vertebrates, recognizes endoplasmic-reticulum-targeted TA proteins and delivers them through a complex series of handoffs. An early step is the formation of a complex between Sgt2/SGTA, a cochaperone with a presumed ubiquitin-like-binding domain (UBD), and Get5/UBL4A, a ubiquitin-like domain (UBL)-containing protein. We structurally characterize this UBD/UBL interaction for both yeast and human proteins. This characterization is supported by biophysical studies that demonstrate that complex formation is mediated by electrostatics, generating an interface that has high-affinity with rapid kinetics. In total, this work provides a refined model of the interplay of Sgt2 homologs in TA targeting.

Structures of the Sgt2/SGTA Dimerization Domain with the Get5/UBL4A UBL Domain Reveal an Interaction that Forms a Conserved Dynamic Interface.,Chartron JW, Vandervelde DG, Clemons WM Jr Cell Rep. 2012 Nov 7. pii: S2211-1247(12)00346-4. doi:, 10.1016/j.celrep.2012.10.010. PMID:23142665[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. Dutta S, Tan YJ. Structural and functional characterization of human SGT and its interaction with Vpu of the human immunodeficiency virus type 1. Biochemistry. 2008 Sep 23;47(38):10123-31. Epub 2008 Aug 30. PMID:18759457 doi:http://dx.doi.org/10.1021/bi800758a
  2. Chartron JW, Vandervelde DG, Clemons WM Jr. Structures of the Sgt2/SGTA Dimerization Domain with the Get5/UBL4A UBL Domain Reveal an Interaction that Forms a Conserved Dynamic Interface. Cell Rep. 2012 Nov 7. pii: S2211-1247(12)00346-4. doi:, 10.1016/j.celrep.2012.10.010. PMID:23142665 doi:http://dx.doi.org/10.1016/j.celrep.2012.10.010

4gof, resolution 1.35Å

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