4hh0: Difference between revisions

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==Dark-state structure of AppA C20S without the Cys-rich region from Rb. sphaeroides==
==Dark-state structure of AppA C20S without the Cys-rich region from Rb. sphaeroides==
<StructureSection load='4hh0' size='340' side='right' caption='[[4hh0]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='4hh0' size='340' side='right'caption='[[4hh0]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4hh0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HH0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HH0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4hh0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HH0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hh1|4hh1]], [[4hh3|4hh3]], [[4hh2|4hh2]], [[2iyg|2iyg]], [[2iyi|2iyi]], [[1yrx|1yrx]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">appA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 "Rhodococcus capsulatus" Molisch 1907])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hh0 OCA], [https://pdbe.org/4hh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hh0 RCSB], [https://www.ebi.ac.uk/pdbsum/4hh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hh0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hh0 OCA], [http://pdbe.org/4hh0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hh0 RCSB], [http://www.ebi.ac.uk/pdbsum/4hh0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hh0 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q53119_CERSP Q53119_CERSP]
The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources, depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their partial expression prepares the bacterium for using light energy. Illumination, however, enhances repression under semiaerobic conditions. Here, we describe molecular details of two proteins mediating oxygen and light control of photosynthesis-gene expression: the light-sensing antirepressor AppA and the transcriptional repressor PpsR. Our crystal structures of both proteins and their complex and hydrogen/deuterium-exchange data show that light activation of AppA-PpsR2 affects the PpsR effector region within the complex. DNA binding studies demonstrate the formation of a light-sensitive ternary AppA-PpsR-DNA complex. We discuss implications of these results for regulation by light and oxygen, highlighting new insights into blue light-mediated signal transduction.


A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression.,Winkler A, Heintz U, Lindner R, Reinstein J, Shoeman RL, Schlichting I Nat Struct Mol Biol. 2013 Jul;20(7):859-67. doi: 10.1038/nsmb.2597. Epub 2013 Jun, 2. PMID:23728293<ref>PMID:23728293</ref>
==See Also==
 
*[[AppA protein BLUF domain|AppA protein BLUF domain]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4hh0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Rhodococcus capsulatus molisch 1907]]
[[Category: Cereibacter sphaeroides]]
[[Category: Heintz, U]]
[[Category: Large Structures]]
[[Category: Lindner, R]]
[[Category: Heintz U]]
[[Category: Reinstein, J]]
[[Category: Lindner R]]
[[Category: Schlichting, I]]
[[Category: Reinstein J]]
[[Category: Shoeman, R]]
[[Category: Schlichting I]]
[[Category: Winkler, A]]
[[Category: Shoeman R]]
[[Category: Bluf domain]]
[[Category: Winkler A]]
[[Category: Flavoprotein]]
[[Category: Photoreceptor]]
[[Category: Ppsr]]
[[Category: Schic domain]]
[[Category: Signaling protein]]

Latest revision as of 14:40, 1 March 2024

Dark-state structure of AppA C20S without the Cys-rich region from Rb. sphaeroidesDark-state structure of AppA C20S without the Cys-rich region from Rb. sphaeroides

Structural highlights

4hh0 is a 2 chain structure with sequence from Cereibacter sphaeroides. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q53119_CERSP

See Also

4hh0, resolution 2.60Å

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