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==Crystal structure of LeuT mutant F259V bound to sodium and L-tryptophan==
==Crystal structure of LeuT mutant F259V bound to sodium and L-tryptophan==
<StructureSection load='3qs4' size='340' side='right' caption='[[3qs4]], [[Resolution|resolution]] 2.63&Aring;' scene=''>
<StructureSection load='3qs4' size='340' side='right'caption='[[3qs4]], [[Resolution|resolution]] 2.63&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3qs4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QS4 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3qs4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QS4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.631&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a65|2a65]], [[3f3a|3f3a]], [[3qs5|3qs5]], [[3qs6|3qs6]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aq_2077, leut, snf ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qs4 OCA], [https://pdbe.org/3qs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qs4 RCSB], [https://www.ebi.ac.uk/pdbsum/3qs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qs4 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qs4 OCA], [http://pdbe.org/3qs4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qs4 RCSB], [http://www.ebi.ac.uk/pdbsum/3qs4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qs4 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/O67854_AQUAE O67854_AQUAE]
LeuT is a bacterial homologue of the neurotransmitter:sodium symporter (NSS) family and, being the only NSS member to have been structurally characterized by X-ray crystallography, is a model protein for studying transporter structure and mechanism. Transport activity in LeuT was hypothesized to require structural transitions between open-to-out and occluded conformations dependent upon protein:ligand binding complementarity. Here, using crystallographic and functional analysis, we show that binding site modification produces changes in both structure and activity that are consistent with complementarity-dependent structural transitions to the occluded state. The mutation I359Q converts the activity of tryptophan from inhibitor to transportable substrate. This mutation changes the local environment of the binding site, inducing the bound tryptophan to adopt a different conformer than in the wild-type complex. Instead of trapping the transporter open, tryptophan binding now allows the formation of an occluded state. Thus, transport activity is correlated to the ability of the ligand to promote the structural transition to the occluded state, a step in the transport cycle that is dependent on protein:ligand complementarity in the central binding site.
 
Insights into transport mechanism from LeuT engineered to transport tryptophan.,Piscitelli CL, Gouaux E EMBO J. 2011 Sep 27. doi: 10.1038/emboj.2011.353. PMID:21952050<ref>PMID:21952050</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3qs4" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Leucine transporter|Leucine transporter]]
*[[Leucine transporter|Leucine transporter]]
== References ==
*[[Symporter 3D structures|Symporter 3D structures]]
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aquifex aeolicus huber and stetter 2001]]
[[Category: Aquifex aeolicus]]
[[Category: Gouaux, E]]
[[Category: Large Structures]]
[[Category: Piscitelli, C L]]
[[Category: Gouaux E]]
[[Category: Leut-fold]]
[[Category: Piscitelli CL]]
[[Category: Membrane]]
[[Category: Neurotransmitter transporter]]
[[Category: Nss]]
[[Category: Sodium and amino acid binding]]
[[Category: Sodium-coupled secondary transporter]]
[[Category: Transport protein]]

Latest revision as of 14:56, 14 March 2024

Crystal structure of LeuT mutant F259V bound to sodium and L-tryptophanCrystal structure of LeuT mutant F259V bound to sodium and L-tryptophan

Structural highlights

3qs4 is a 1 chain structure with sequence from Aquifex aeolicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.631Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O67854_AQUAE

See Also

3qs4, resolution 2.63Å

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