4j89: Difference between revisions

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==Different photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescence==
==Different photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescence==
<StructureSection load='4j89' size='340' side='right' caption='[[4j89]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='4j89' size='340' side='right'caption='[[4j89]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4j89]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J89 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J89 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4j89]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J89 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J89 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CQ2:{(4Z)-4-(4-AMINOBENZYLIDENE)-2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CQ2</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CQ2:{(4Z)-4-(4-AMINOBENZYLIDENE)-2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CQ2</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4j88|4j88]], [[4j8a|4j8a]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j89 OCA], [https://pdbe.org/4j89 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j89 RCSB], [https://www.ebi.ac.uk/pdbsum/4j89 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j89 ProSAT]</span></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j89 OCA], [http://pdbe.org/4j89 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j89 RCSB], [http://www.ebi.ac.uk/pdbsum/4j89 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j89 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.  
[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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==See Also==
==See Also==
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aeqvi]]
[[Category: Aequorea victoria]]
[[Category: Jones, D D]]
[[Category: Large Structures]]
[[Category: Reddington, S C]]
[[Category: Jones DD]]
[[Category: Rizkallah, P J]]
[[Category: Reddington SC]]
[[Category: Tippmann, E M]]
[[Category: Rizkallah PJ]]
[[Category: Beta-barrel]]
[[Category: Tippmann EM]]
[[Category: Chromophore by cyclisation]]
[[Category: Cytosol]]
[[Category: Fluorescent protein]]
[[Category: P-azido-l-phenylalanine]]

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