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[[Image:1jt0.gif|left|200px]]


{{Structure
==Crystal structure of a cooperative QacR-DNA complex==
|PDB= 1jt0 |SIZE=350|CAPTION= <scene name='initialview01'>1jt0</scene>, resolution 2.90&Aring;
<StructureSection load='1jt0' size='340' side='right'caption='[[1jt0]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
<table><tr><td colspan='2'>[[1jt0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JT0 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt0 OCA], [https://pdbe.org/1jt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jt0 RCSB], [https://www.ebi.ac.uk/pdbsum/1jt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt0 ProSAT]</span></td></tr>
|RELATEDENTRY=[[1jt6|1JT6]], [[1jty|1JTY]], [[1jum|1JUM]], [[1jup|1JUP]], [[1jus|1JUS]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt0 OCA], [http://www.ebi.ac.uk/pdbsum/1jt0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jt0 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/QACR_STAAU QACR_STAAU] Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA.
 
== Evolutionary Conservation ==
'''Crystal structure of a cooperative QacR-DNA complex'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jt0_consurf.spt"</scriptWhenChecked>
The Staphylococcus aureus multidrug-binding protein QacR represses transcription of the qacA multidrug transporter gene and is induced by multiple structurally dissimilar drugs. QacR is a member of the TetR/CamR family of transcriptional regulators, which share highly homologous N-terminal DNA-binding domains connected to seemingly non-homologous ligand-binding domains. Unlike other TetR members, which bind approximately 15 bp operators, QacR recognizes an unusually long 28 bp operator, IR1, which it appears to bind cooperatively. To elucidate the DNA-binding mechanism of QacR, we determined the 2.90 A resolution crystal structure of a QacR-IR1 complex. Strikingly, our data reveal that the DNA recognition mode of QacR is distinct from TetR and involves the binding of a pair of QacR dimers. In this unique binding mode, recognition at each IR1 half-site is mediated by a complement of DNA contacts made by two helix-turn-helix motifs. The inferred cooperativity does not arise from cross-dimer protein-protein contacts, but from the global undertwisting and major groove widening elicited by the binding of two QacR dimers.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1JT0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT0 OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jt0 ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR., Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG, EMBO J. 2002 Mar 1;21(5):1210-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11867549 11867549]
</StructureSection>
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
[[Category: Brennan, R G.]]
[[Category: Brennan RG]]
[[Category: Brown, M H.]]
[[Category: Brown MH]]
[[Category: Grkovic, S.]]
[[Category: Grkovic S]]
[[Category: Miller, M C.]]
[[Category: Miller MC]]
[[Category: Schumacher, M A.]]
[[Category: Schumacher MA]]
[[Category: Skurray, R A.]]
[[Category: Skurray RA]]
[[Category: cooperative dna binding]]
[[Category: dimer of dimer]]
[[Category: multidrug binding protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:38:34 2008''

Latest revision as of 10:42, 7 February 2024

Crystal structure of a cooperative QacR-DNA complexCrystal structure of a cooperative QacR-DNA complex

Structural highlights

1jt0 is a 6 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.9Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

QACR_STAAU Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1jt0, resolution 2.90Å

Drag the structure with the mouse to rotate

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OCA
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