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| [[Image:1huf.jpg|left|200px]]
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| {{Structure
| | ==CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF THE TYROSINE PHOSPHATASE YOPH FROM YERSINIA PESTIS.== |
| |PDB= 1huf |SIZE=350|CAPTION= <scene name='initialview01'>1huf</scene>, resolution 2.0Å
| | <StructureSection load='1huf' size='340' side='right'caption='[[1huf]], [[Resolution|resolution]] 2.00Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[1huf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HUF FirstGlance]. <br> |
| |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span>
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| |GENE= YOPH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis])
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1huf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1huf OCA], [https://pdbe.org/1huf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1huf RCSB], [https://www.ebi.ac.uk/pdbsum/1huf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1huf ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=[[1ypt|1YPT]]
| | == Function == |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1huf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1huf OCA], [http://www.ebi.ac.uk/pdbsum/1huf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1huf RCSB]</span>
| | [https://www.uniprot.org/uniprot/O68720_YERPE O68720_YERPE] |
| }}
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| '''CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF THE TYROSINE PHOSPHATASE YOPH FROM YERSINIA PESTIS.'''
| | ==See Also== |
| | | *[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| Yersinia pestis, the causative agent of bubonic plague, injects effector proteins into the cytosol of mammalian cells that enable the bacterium to evade the immune response of the infected organism by interfering with eukaryotic signal transduction pathways. YopH is a modular effector composed of a C-terminal protein tyrosine phosphatase (PTPase) domain and a multifunctional N-terminal domain that not only orchestrates the secretion and translocation of YopH into eukaryotic cells but also binds tyrosine-phosphorylated target proteins to mediate substrate recognition. The crystal structure of the N-terminal domain of YopH (YopH(N); residues 1-130) has been determined at 2.0 A resolution. The amino-acid sequences that target YopH for secretion from the bacterium and translocation into eukaryotic cells form integral parts of this compactly folded domain. The structure of YopH(N) bears no resemblance to eukaryotic phosphotyrosine-binding domains, nor is it reminiscent of any known fold. Residues that have been implicated in phosphotyrosine-dependent protein binding are clustered together on one face of YopH(N), but the structure does not suggest a mechanism for protein-phosphotyrosine recognition.
| | [[Category: Large Structures]] |
| | |
| ==About this Structure==
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| 1HUF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HUF OCA].
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| ==Reference==
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| Structure of the N-terminal domain of Yersinia pestis YopH at 2.0 A resolution., Evdokimov AG, Tropea JE, Routzahn KM, Copeland TD, Waugh DS, Acta Crystallogr D Biol Crystallogr. 2001 Jun;57(Pt 6):793-9. Epub 2001, May 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11375498 11375498]
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| [[Category: Protein-tyrosine-phosphatase]]
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| [[Category: Single protein]] | |
| [[Category: Yersinia pestis]] | | [[Category: Yersinia pestis]] |
| [[Category: Evdokimov, A G.]] | | [[Category: Evdokimov AG]] |
| [[Category: Waugh, D S.]] | | [[Category: Waugh DS]] |
| [[Category: beta hairpin]]
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| [[Category: helical bundle]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:10:30 2008''
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