5i6c: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
==The structure of the eukaryotic purine/H+ symporter, UapA, in complex with Xanthine== | ==The structure of the eukaryotic purine/H+ symporter, UapA, in complex with Xanthine== | ||
<StructureSection load='5i6c' size='340' side='right' caption='[[5i6c]], [[Resolution|resolution]] 3.70Å' scene=''> | <StructureSection load='5i6c' size='340' side='right'caption='[[5i6c]], [[Resolution|resolution]] 3.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5i6c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I6C OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5i6c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I6C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I6C FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=XAN:XANTHINE'>XAN</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=XAN:XANTHINE'>XAN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i6c OCA], [https://pdbe.org/5i6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i6c RCSB], [https://www.ebi.ac.uk/pdbsum/5i6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i6c ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/UAPA_EMENI UAPA_EMENI] Uric acid-xanthine transporter.<ref>PMID:15953615</ref> <ref>PMID:16096268</ref> <ref>PMID:20002879</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 18: | Line 19: | ||
</div> | </div> | ||
<div class="pdbe-citations 5i6c" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5i6c" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Symporter 3D structures|Symporter 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Alguel | [[Category: Aspergillus nidulans FGSC A4]] | ||
[[Category: Amillis | [[Category: Large Structures]] | ||
[[Category: Armstrong | [[Category: Alguel Y]] | ||
[[Category: Byrne | [[Category: Amillis S]] | ||
[[Category: Cameron | [[Category: Armstrong A]] | ||
[[Category: Capaldi | [[Category: Byrne B]] | ||
[[Category: Craven | [[Category: Cameron AD]] | ||
[[Category: Diallinas | [[Category: Capaldi S]] | ||
[[Category: Iwata | [[Category: Craven G]] | ||
[[Category: Lambrinidis | [[Category: Diallinas G]] | ||
[[Category: Leung | [[Category: Iwata S]] | ||
[[Category: Mikros | [[Category: Lambrinidis G]] | ||
[[Category: Scull | [[Category: Leung J]] | ||
[[Category: Mikros E]] | |||
[[Category: Scull NJ]] | |||
Latest revision as of 11:24, 23 August 2023
The structure of the eukaryotic purine/H+ symporter, UapA, in complex with XanthineThe structure of the eukaryotic purine/H+ symporter, UapA, in complex with Xanthine
Structural highlights
FunctionUAPA_EMENI Uric acid-xanthine transporter.[1] [2] [3] Publication Abstract from PubMedThe uric acid/xanthine H(+) symporter, UapA, is a high-affinity purine transporter from the filamentous fungus Aspergillus nidulans. Here we present the crystal structure of a genetically stabilized version of UapA (UapA-G411VDelta1-11) in complex with xanthine. UapA is formed from two domains, a core domain and a gate domain, similar to the previously solved uracil transporter UraA, which belongs to the same family. The structure shows UapA in an inward-facing conformation with xanthine bound to residues in the core domain. Unlike UraA, which was observed to be a monomer, UapA forms a dimer in the crystals with dimer interactions formed exclusively through the gate domain. Analysis of dominant negative mutants is consistent with dimerization playing a key role in transport. We postulate that UapA uses an elevator transport mechanism likely to be shared with other structurally homologous transporters including anion exchangers and prestin. Structure of eukaryotic purine/H(+) symporter UapA suggests a role for homodimerization in transport activity.,Alguel Y, Amillis S, Leung J, Lambrinidis G, Capaldi S, Scull NJ, Craven G, Iwata S, Armstrong A, Mikros E, Diallinas G, Cameron AD, Byrne B Nat Commun. 2016 Apr 18;7:11336. doi: 10.1038/ncomms11336. PMID:27088252[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||