NAD synthase: Difference between revisions
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<StructureSection load='' size='350' side='right' caption='NAD+ synthase complex with Mg+2 (green), ATP and deamino-NAD (PDB entry [[1xng]])' scene='54/541072/Cv/1'> | |||
== Function == | |||
'''NAD+ synthase''' or '''NH3-dependent NAD synthetase''' (NADS) catalyzes the conversion of ATP, deamino-NAD+ and NH<sub>3</sub> to AMP, NAD+ and diphosphate (PP). NADS is part of the NAD synthesis pathway<ref>PMID:12898714</ref> . See [[NAD]]. | |||
'''NAD | |||
== Structural highlights == | |||
The <scene name='54/541072/Cv/8'>ATP binding site</scene> contains <scene name='54/541072/Cv/9'>Mg+2 ion</scene>. The <scene name='54/541072/Cv/10'>NAD binding site is located in the interface of the 2 subunits</scene><ref>PMID:15645437</ref>. Water molecules are shown as red spheres. | |||
</StructureSection> | |||
== 3D Structures of NAD+ synthase == | == 3D Structures of NAD+ synthase == | ||
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**[[2pzb]] – BaNADS - ''Bacillus anthracis'' <br /> | **[[2pzb]] – BaNADS - ''Bacillus anthracis'' <br /> | ||
**[[3dpi]] – NADS – ''Burkholderia pseudomallei''<br /> | **[[3dpi]] – NADS – ''Burkholderia pseudomallei''<br /> | ||
**[[6kv3]] – NADS – ''Staphylococcus aureus''<br /> | |||
**[[5wp0]] – NADS – ''Allivibrio fischeri''<br /> | |||
**[[5huh]], [[5huj]] – NADS – ''Streptococcus pyogenes''<br /> | |||
**[[3n05]] – NADS – ''Streptomyces avermitillis''<br /> | |||
**[[4xfd]] – PaNADS – ''Pseudomonas aeruginosa''<br /> | |||
**[[4q16]] – NADS – ''Deinococcus radiodurans''<br /> | |||
**[[2e18]] – NADS – ''Pyrococcus horikoshii''<br /> | |||
*NADS binary complex | *NADS binary complex | ||
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**[[1wxh]] – EcNADS + NAD <br /> | **[[1wxh]] – EcNADS + NAD <br /> | ||
**[[3hmq]] – BsNADS + NAD – ''Salmonella typhimurium'' <br /> | **[[3hmq]] – BsNADS + NAD – ''Salmonella typhimurium'' <br /> | ||
**[[6c8q]] – NADS + NAD – ''Enterococcus faecalis''<br /> | |||
**[[5f23]] – PaNADS + NAD <br /> | |||
*NADS ternary complex | *NADS ternary complex | ||
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**[[1wxg]] – EcNADS + Mg + deamino-NAD <br /> | **[[1wxg]] – EcNADS + Mg + deamino-NAD <br /> | ||
**[[2pz8]] – BaNADS + Mg + ATP derivative <br /> | **[[2pz8]] – BaNADS + Mg + ATP derivative <br /> | ||
**[[3fiu]] – NADS + Mg + AMP – ''Francisella tularensis''<br /> | |||
*NADS quaternary complex | *NADS quaternary complex | ||
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*NADS glutamine-dependent | *NADS glutamine-dependent | ||
**[[6ofb]] – hNADS + AMP + NAD derivative<br /> | |||
**[[3sdb]] – MtNADS (mutant) – ''Mycobacterium tuberculosis'' <br /> | **[[3sdb]] – MtNADS (mutant) – ''Mycobacterium tuberculosis'' <br /> | ||
**[[3seq]], [[3sez]] – MtNADS (mutant) + ATP derivative + pyridinium derivative <br /> | **[[3seq]], [[3sez]] – MtNADS (mutant) + ATP derivative + pyridinium derivative <br /> | ||
**[[3szg]] – MtNADS (mutant) + AMP + pyridinium derivative <br /> | **[[3szg]] – MtNADS (mutant) + AMP + pyridinium derivative <br /> | ||
**[[3syt]] – MtNADS + AMP + NAD + glutamate <br /> | **[[3syt]] – MtNADS + AMP + NAD + glutamate <br /> | ||
**[[3dla]] – MtNADS + norleucine + deamido-NAD <br /> | |||
**[[6ofc]] – MtNADS (mutant) + AMP + sulfonamide derivative + glutamate <br /> | |||
**[[4f4h]] – NADS – ''Burkholderia thailandensis''<br /> | |||
**[[3ilv]] – NADS – ''Cytophaga hutchinsonii''<br /> | |||
}} | }} | ||