1h9h: Difference between revisions

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-[[Image:1h9h.jpg|left|200px]]
- {{Structure
+==COMPLEX OF EETI-II WITH PORCINE TRYPSIN==
-|PDB= 1h9h |SIZE=350|CAPTION= <scene name='initialview01'>1h9h</scene>, resolution 1.5&Aring;
+<StructureSection load='1h9h' size='340' side='right'caption='[[1h9h]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
-|SITE= <scene name='pdbsite=CA1:Ca+Binding+Site+For+Chain+E'>CA1</scene>
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
+<table><tr><td colspan='2'>[[1h9h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecballium_elaterium Ecballium elaterium] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H9H FirstGlance]. <br>
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9h OCA], [https://pdbe.org/1h9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h9h RCSB], [https://www.ebi.ac.uk/pdbsum/1h9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h9h ProSAT]</span></td></tr>
-|RELATEDENTRY=
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9h OCA], [http://www.ebi.ac.uk/pdbsum/1h9h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1h9h RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/TRYP_PIG TRYP_PIG]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h9/1h9h_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h9h ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold.
-'''COMPLEX OF EETI-II WITH PORCINE TRYPSIN'''
+Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold.,Kratzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1255-62. Epub 2005, Aug 16. PMID:16131759<ref>PMID:16131759</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1h9h" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-H9H is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Ecballium_elaterium Ecballium elaterium] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9H OCA].
+*[[Trypsin 3D structures|Trypsin 3D structures]]
+*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Ecballium elaterium]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
 [[Category: Sus scrofa]]
-[[Category: Trypsin]]
+[[Category: Kolmar H]]
-[[Category: Kolmar, H.]]
+[[Category: Kraetzner R]]
-[[Category: Kraetzner, R.]]
+[[Category: Uson I]]
-[[Category: Uson, I.]]
+[[Category: Wentzel A]]
-[[Category: Wentzel, A.]]
-[[Category: cystine knot]]
-[[Category: squash inhibitor]]
-[[Category: trypsin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:00:31 2008''