5hr0: Difference between revisions

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-'''Unreleased structure'''
-The entry 5hr0 is ON HOLD  until Paper Publication
+==Crystal structure of thioredoxin E101G mutant==
+<StructureSection load='5hr0' size='340' side='right'caption='[[5hr0]], [[Resolution|resolution]] 1.31&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5hr0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HR0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.31&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hr0 OCA], [https://pdbe.org/5hr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hr0 RCSB], [https://www.ebi.ac.uk/pdbsum/5hr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hr0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/THIO_ECOLI THIO_ECOLI] Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Thioredoxin is a ubiquitous small protein that catalyzes redox reactions of protein thiols. Additionally, thioredoxin from E. coli (EcTRX) is a widely-used model for structure-function studies. In a previous paper, we characterized several single-point mutants of the C-terminal helix (CTH) that alter global stability of EcTRX. However, spectroscopic signatures and enzymatic activity for some of these mutants were found essentially unaffected. A comprehensive structural characterization at the atomic level of these near-invariant mutants can provide detailed information about structural variability of EcTRX. We address this point through the determination of the crystal structures of four point-mutants, whose mutations occurs within or near the CTH, namely L94A, E101G, N106A and L107A. These structures are mostly unaffected compared with the wild-type variant. Notably, the E101G mutant presents a large region with two alternative traces for the backbone of the same chain. It represents a significant shift in backbone positions. Enzymatic activity measurements and conformational dynamics studies monitored by NMR and molecular dynamic simulations show that E101G mutation results in a small effect in the structural features of the protein. We hypothesize that these alternative conformations represent samples of the native-state ensemble of EcTRX, specifically the magnitude and location of conformational heterogeneity.
-Authors: Noguera, M.E., Vazquez, D.S., Howard, E.I., Cousido-Siah, A., Mitschler, A., Podjarny, A., Santos, J.
+Structural variability of E. coli thioredoxin captured in the crystal structures of single-point mutants.,Noguera ME, Vazquez DS, Ferrer-Sueta G, Agudelo WA, Howard E, Rasia RM, Manta B, Cousido-Siah A, Mitschler A, Podjarny A, Santos J Sci Rep. 2017 Feb 9;7:42343. doi: 10.1038/srep42343. PMID:28181556<ref>PMID:28181556</ref>
-Description: Crystal structure of thioredoxin E101G mutant
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Vazquez, D.S]]
+<div class="pdbe-citations 5hr0" style="background-color:#fffaf0;"></div>
-[[Category: Noguera, M.E]]
-[[Category: Podjarny, A]]
+==See Also==
-[[Category: Howard, E.I]]
+*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
-[[Category: Mitschler, A]]
+== References ==
-[[Category: Santos, J]]
+<references/>
-[[Category: Cousido-Siah, A]]
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli]]
+[[Category: Large Structures]]
+[[Category: Cousido-Siah A]]
+[[Category: Howard EI]]
+[[Category: Mitschler A]]
+[[Category: Noguera ME]]
+[[Category: Podjarny A]]
+[[Category: Santos J]]
+[[Category: Vazquez DS]]