1eof: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
==CRYSTAL STRUCTURE OF THE N136A MUTANT OF A SHAKER T1 DOMAIN==
==CRYSTAL STRUCTURE OF THE N136A MUTANT OF A SHAKER T1 DOMAIN==
<StructureSection load='1eof' size='340' side='right' caption='[[1eof]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
<StructureSection load='1eof' size='340' side='right'caption='[[1eof]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1eof]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aplca Aplca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EOF FirstGlance]. <br>
<table><tr><td colspan='2'>[[1eof]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EOF FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1eod|1eod]], [[1eoe|1eoe]], [[1t1d|1t1d]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eof OCA], [http://pdbe.org/1eof PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eof RCSB], [http://www.ebi.ac.uk/pdbsum/1eof PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eof OCA], [https://pdbe.org/1eof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eof RCSB], [https://www.ebi.ac.uk/pdbsum/1eof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eof ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q16968_APLCA Q16968_APLCA]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/1eof_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/1eof_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
Line 16: Line 19:
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eof ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eof ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The T1 domain, a highly conserved cytoplasmic portion at the N-terminus of the voltage-dependent K+ channel (Kv) alpha-subunit, is responsible for driving and regulating the tetramerization of the alpha-subunits. Here we report the identification of a set of mutations in the T1 domain that alter the gating properties of the Kv channel. Two mutants produce a leftward shift in the activation curve and slow the channel closing rate while a third mutation produces a rightward shift in the activation curve and speeds the channel closing rate. We have determined the crystal structures of T1 domains containing these mutations. Both of the leftward shifting mutants produce similar conformational changes in the putative membrane facing surface of the T1 domain. These results suggest that the structure of the T1 domain in this region is tightly coupled to the channel's gating states.
Voltage dependent activation of potassium channels is coupled to T1 domain structure.,Cushman SJ, Nanao MH, Jahng AW, DeRubeis D, Choe S, Pfaffinger PJ Nat Struct Biol. 2000 May;7(5):403-7. PMID:10802739<ref>PMID:10802739</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1eof" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Potassium Channel|Potassium Channel]]
*[[Potassium channel 3D structures|Potassium channel 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aplca]]
[[Category: Aplysia californica]]
[[Category: Choe, S]]
[[Category: Large Structures]]
[[Category: Cushman, S J]]
[[Category: Choe S]]
[[Category: DeRubeis, D]]
[[Category: Cushman SJ]]
[[Category: Jahng, A W]]
[[Category: DeRubeis D]]
[[Category: Nanao, M H]]
[[Category: Jahng AW]]
[[Category: Pfaffinger, P J]]
[[Category: Nanao MH]]
[[Category: Aplysia kv1 1]]
[[Category: Pfaffinger PJ]]
[[Category: Membrane protein]]
[[Category: Potassium channel]]
[[Category: Proton transport]]

Latest revision as of 10:05, 7 February 2024

CRYSTAL STRUCTURE OF THE N136A MUTANT OF A SHAKER T1 DOMAINCRYSTAL STRUCTURE OF THE N136A MUTANT OF A SHAKER T1 DOMAIN

Structural highlights

1eof is a 1 chain structure with sequence from Aplysia californica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.38Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q16968_APLCA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1eof, resolution 2.38Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1eof&oldid=4042788"