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| [[Image:1duq.gif|left|200px]]
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| {{Structure
| | ==CRYSTAL STRUCTURE OF THE REV BINDING ELEMENT OF HIV-1== |
| |PDB= 1duq |SIZE=350|CAPTION= <scene name='initialview01'>1duq</scene>, resolution 2.10Å
| | <StructureSection load='1duq' size='340' side='right'caption='[[1duq]], [[Resolution|resolution]] 2.10Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| | <table><tr><td colspan='2'>[[1duq]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DUQ FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1duq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1duq OCA], [https://pdbe.org/1duq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1duq RCSB], [https://www.ebi.ac.uk/pdbsum/1duq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1duq ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1duq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1duq OCA], [http://www.ebi.ac.uk/pdbsum/1duq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1duq RCSB]</span>
| | __TOC__ |
| }}
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| '''CRYSTAL STRUCTURE OF THE REV BINDING ELEMENT OF HIV-1'''
| | [[Category: Holbrook EL]] |
| | | [[Category: Holbrook SR]] |
| | | [[Category: Hung L-W]] |
| ==Overview==
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| The crystal and molecular structure of an RNA duplex corresponding to the high affinity Rev protein binding element (RBE) has been determined at 2.1-A resolution. Four unique duplexes are present in the crystal, comprising two structural variants. In each duplex, the RNA double helix consists of an annealed 12-mer and 14-mer that form an asymmetric internal loop consisting of G-G and G-A noncanonical base pairs and a flipped-out uridine. The 12-mer strand has an A-form conformation, whereas the 14-mer strand is distorted to accommodate the bulges and noncanonical base pairing. In contrast to the NMR model of the unbound RBE, an asymmetric G-G pair with N2-N7 and N1-O6 hydrogen bonding, is formed in each helix. The G-A base pairing agrees with the NMR structure in one structural variant, but forms a novel water-mediated pair in the other. A backbone flip and reorientation of the G-G base pair is required to assume the RBE conformation present in the NMR model of the complex between the RBE and the Rev peptide.
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| ==About this Structure==
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| 1DUQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUQ OCA].
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| ==Reference==
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| The crystal structure of the Rev binding element of HIV-1 reveals novel base pairing and conformational variability., Hung LW, Holbrook EL, Holbrook SR, Proc Natl Acad Sci U S A. 2000 May 9;97(10):5107-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10792052 10792052]
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| [[Category: Protein complex]] | |
| [[Category: Holbrook, E L.]] | |
| [[Category: Holbrook, S R.]] | |
| [[Category: Hung, L W.]] | |
| [[Category: hiv-1]]
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| [[Category: rev binding domain]]
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| [[Category: rre]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:49:03 2008''
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