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==Crystal of Selenomethionine substituted AmtR from Corynebacterium glutamicum== | ==Crystal of Selenomethionine substituted AmtR from Corynebacterium glutamicum== | ||
<StructureSection load='5dy1' size='340' side='right' caption='[[5dy1]], [[Resolution|resolution]] 2.65Å' scene=''> | <StructureSection load='5dy1' size='340' side='right'caption='[[5dy1]], [[Resolution|resolution]] 2.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5dy1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DY1 OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5dy1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum_ATCC_14067 Corynebacterium glutamicum ATCC 14067]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DY1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DY1 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.651Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dy1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dy1 OCA], [https://pdbe.org/5dy1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dy1 RCSB], [https://www.ebi.ac.uk/pdbsum/5dy1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dy1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Corynebacterium glutamicum is a bacterium used for industrial amino acid production and understanding its metabolic pathway regulation is of high biotechnological interest. Here, we report crystal structures of AmtR, the global nitrogen regulator of C. glutamicum, in apo (2.25A and 2.65A resolution) and DNA-bound (3A resolution) forms. These structures reveal an all-alpha homodimeric TetR family regulator composed of a helix-turn-helix-hosting N-terminal DNA binding domain and a C-terminal dimerization domain. AmtR presents several unique structural features that appear invariant among AmtR proteins, which may be related to its regulation by the nitrogen-sensing trimeric protein GlnK rather than by small molecule effectors. Compared with other TetR family members, AmtR has an extra C-terminal helix, a large extended external loop that resembles the flexible tranducer T-loop of GlnK in sequence, and a large open cavity towards the intersubunit region that changes shape upon DNA binding. The marked kinking of helix 4 decreases in the DNA-bound form. The binding of one AmtR dimer to its DNA operator not only involves the insertion of helices 3 and 3' in adjacent turns of the double-helix major groove, but also the anchoring of 19-residue, arginine-rich and proline-rich N-terminal extensions to two external minor grooves. EMSA assays with a deletion mutant reveal that the 19-residue extension is crucial for AmtR binding to DNA. N-Extension anchoring explains the flanking by AT sequences of the recognized target DNA sequence core. The significance of these findings for the entire TetR family of regulators and for GlnK regulation of AmtR is discussed. This article is protected by copyright. All rights reserved. | |||
Structure of AmtR, the global nitrogen regulator of Corynebacterium glutamicum, in free and DNA-bound forms.,Palanca C, Rubio V FEBS J. 2016 Jan 7. doi: 10.1111/febs.13643. PMID:26744254<ref>PMID:26744254</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5dy1" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Corynebacterium glutamicum ATCC 14067]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Palanca C]] | ||
[[Category: | [[Category: Rubio V]] | ||
Latest revision as of 01:05, 29 June 2023
Crystal of Selenomethionine substituted AmtR from Corynebacterium glutamicumCrystal of Selenomethionine substituted AmtR from Corynebacterium glutamicum
Structural highlights
Publication Abstract from PubMedCorynebacterium glutamicum is a bacterium used for industrial amino acid production and understanding its metabolic pathway regulation is of high biotechnological interest. Here, we report crystal structures of AmtR, the global nitrogen regulator of C. glutamicum, in apo (2.25A and 2.65A resolution) and DNA-bound (3A resolution) forms. These structures reveal an all-alpha homodimeric TetR family regulator composed of a helix-turn-helix-hosting N-terminal DNA binding domain and a C-terminal dimerization domain. AmtR presents several unique structural features that appear invariant among AmtR proteins, which may be related to its regulation by the nitrogen-sensing trimeric protein GlnK rather than by small molecule effectors. Compared with other TetR family members, AmtR has an extra C-terminal helix, a large extended external loop that resembles the flexible tranducer T-loop of GlnK in sequence, and a large open cavity towards the intersubunit region that changes shape upon DNA binding. The marked kinking of helix 4 decreases in the DNA-bound form. The binding of one AmtR dimer to its DNA operator not only involves the insertion of helices 3 and 3' in adjacent turns of the double-helix major groove, but also the anchoring of 19-residue, arginine-rich and proline-rich N-terminal extensions to two external minor grooves. EMSA assays with a deletion mutant reveal that the 19-residue extension is crucial for AmtR binding to DNA. N-Extension anchoring explains the flanking by AT sequences of the recognized target DNA sequence core. The significance of these findings for the entire TetR family of regulators and for GlnK regulation of AmtR is discussed. This article is protected by copyright. All rights reserved. Structure of AmtR, the global nitrogen regulator of Corynebacterium glutamicum, in free and DNA-bound forms.,Palanca C, Rubio V FEBS J. 2016 Jan 7. doi: 10.1111/febs.13643. PMID:26744254[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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