4rzs: Difference between revisions

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==Lac repressor engineered to bind sucralose, unliganded tetramer==
==Lac repressor engineered to bind sucralose, unliganded tetramer==
<StructureSection load='4rzs' size='340' side='right' caption='[[4rzs]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
<StructureSection load='4rzs' size='340' side='right'caption='[[4rzs]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4rzs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RZS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RZS FirstGlance]. <br>
<table><tr><td colspan='2'>[[4rzs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH1 Escherichia coli DH1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RZS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.71&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzs OCA], [http://pdbe.org/4rzs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rzs RCSB], [http://www.ebi.ac.uk/pdbsum/4rzs PDBsum]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzs OCA], [https://pdbe.org/4rzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rzs RCSB], [https://www.ebi.ac.uk/pdbsum/4rzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rzs ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
Genetic regulatory proteins inducible by small molecules are useful synthetic biology tools as sensors and switches. Bacterial allosteric transcription factors (aTFs) are a major class of regulatory proteins, but few aTFs have been redesigned to respond to new effectors beyond natural aTF-inducer pairs. Altering inducer specificity in these proteins is difficult because substitutions that affect inducer binding may also disrupt allostery. We engineered an aTF, the Escherichia coli lac repressor, LacI, to respond to one of four new inducer molecules: fucose, gentiobiose, lactitol and sucralose. Using computational protein design, single-residue saturation mutagenesis or random mutagenesis, along with multiplex assembly, we identified new variants comparable in specificity and induction to wild-type LacI with its inducer, isopropyl beta-D-1-thiogalactopyranoside (IPTG). The ability to create designer aTFs will enable applications including dynamic control of cell metabolism, cell biology and synthetic gene circuits.
 
Engineering an allosteric transcription factor to respond to new ligands.,Taylor ND, Garruss AS, Moretti R, Chan S, Arbing MA, Cascio D, Rogers JK, Isaacs FJ, Kosuri S, Baker D, Fields S, Church GM, Raman S Nat Methods. 2015 Dec 21. doi: 10.1038/nmeth.3696. PMID:26689263<ref>PMID:26689263</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[Lac repressor|Lac repressor]]
<div class="pdbe-citations 4rzs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Arbing, M A]]
[[Category: Escherichia coli DH1]]
[[Category: Cascio, D]]
[[Category: Large Structures]]
[[Category: Church, G M]]
[[Category: Arbing MA]]
[[Category: Kosuri, S]]
[[Category: Cascio D]]
[[Category: Allostery]]
[[Category: Church GM]]
[[Category: Escherichia coli]]
[[Category: Kosuri S]]
[[Category: Lac repressor]]
[[Category: Laci]]
[[Category: Lactose operon repressor]]
[[Category: Protein design]]
[[Category: Sucralose]]
[[Category: Transcription]]

Latest revision as of 15:57, 1 March 2024

Lac repressor engineered to bind sucralose, unliganded tetramerLac repressor engineered to bind sucralose, unliganded tetramer

Structural highlights

4rzs is a 4 chain structure with sequence from Escherichia coli DH1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.71Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LACI_ECOLI Repressor of the lactose operon. Binds allolactose as an inducer.

See Also

4rzs, resolution 2.71Å

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