5foo: Difference between revisions

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'''Unreleased structure'''


The entry 5foo is ON HOLD  until Paper Publication
==6-phospho-beta-glucosidase==
<StructureSection load='5foo' size='340' side='right'caption='[[5foo]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5foo]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_M1_GAS Streptococcus pyogenes M1 GAS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FOO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=JAZ:6-PHOSPHOCYCLOPHELLITOL'>JAZ</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5foo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5foo OCA], [https://pdbe.org/5foo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5foo RCSB], [https://www.ebi.ac.uk/pdbsum/5foo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5foo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q99YP9_STRP1 Q99YP9_STRP1]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-beta-glucosidase from CAZy family GH1. We further present the three-dimensional structure of the inactivated enzyme, which reveals the constellation of active site residues responsible for the enzyme's specificity and confirms the covalent nature of the inactivation.


Authors: Jin, Y., Kwan, D.H., Withers, S.G., Davies, G.J.
Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-beta-glucosidases.,Kwan DH, Jin Y, Jiang J, Chen HM, Kotzler MP, Overkleeft HS, Davies GJ, Withers SG FEBS Lett. 2016 Feb;590(4):461-8. doi: 10.1002/1873-3468.12059. Epub 2016 Feb 14. PMID:26790390<ref>PMID:26790390</ref>


Description: 6-phospho-beta-glucosidase
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Davies, G.J]]
<div class="pdbe-citations 5foo" style="background-color:#fffaf0;"></div>
[[Category: Withers, S.G]]
 
[[Category: Kwan, D.H]]
==See Also==
[[Category: Jin, Y]]
*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pyogenes M1 GAS]]
[[Category: Davies GJ]]
[[Category: Jin Y]]
[[Category: Kwan DH]]
[[Category: Withers SG]]

Latest revision as of 10:01, 19 July 2023

6-phospho-beta-glucosidase6-phospho-beta-glucosidase

Structural highlights

5foo is a 6 chain structure with sequence from Streptococcus pyogenes M1 GAS. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:, , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q99YP9_STRP1

Publication Abstract from PubMed

Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-beta-glucosidase from CAZy family GH1. We further present the three-dimensional structure of the inactivated enzyme, which reveals the constellation of active site residues responsible for the enzyme's specificity and confirms the covalent nature of the inactivation.

Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-beta-glucosidases.,Kwan DH, Jin Y, Jiang J, Chen HM, Kotzler MP, Overkleeft HS, Davies GJ, Withers SG FEBS Lett. 2016 Feb;590(4):461-8. doi: 10.1002/1873-3468.12059. Epub 2016 Feb 14. PMID:26790390[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Kwan DH, Jin Y, Jiang J, Chen HM, Kotzler MP, Overkleeft HS, Davies GJ, Withers SG. Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-beta-glucosidases. FEBS Lett. 2016 Feb;590(4):461-8. doi: 10.1002/1873-3468.12059. Epub 2016 Feb 14. PMID:26790390 doi:http://dx.doi.org/10.1002/1873-3468.12059

5foo, resolution 2.10Å

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