2bl2: Difference between revisions

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-[[Image:2bl2.gif|left|200px]]<br />
-<applet load="2bl2" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2bl2, resolution 2.10&Aring;" />
-'''THE MEMBRANE ROTOR OF THE V-TYPE ATPASE FROM ENTEROCOCCUS HIRAE'''<br />
-==Overview==
+==The membrane rotor of the V-type ATPase from Enterococcus hirae==
-The membrane rotor ring from the vacuolar-type (V-type) sodium ion-pumping, adenosine triphosphatase (Na+-ATPase) from Enterococcus hirae consists of, 10 NtpK subunits, which are homologs of the 16-kilodalton and 8-kilodalton, proteolipids found in other V-ATPases and in F1Fo- or F-ATPases, respectively. Each NtpK subunit has four transmembrane alpha helices, with, a sodium ion bound between helices 2 and 4 at a site buried deeply in the, membrane that includes the essential residue glutamate-139. This site is, probably connected to the membrane surface by two half-channels in subunit, NtpI, against which the ring rotates. Symmetry mismatch between the rotor, and catalytic domains appears to be an intrinsic feature of both V- and, F-ATPases.
+<StructureSection load='2bl2' size='340' side='right'caption='[[2bl2]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2bl2]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_hirae Enterococcus hirae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BL2 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=UMQ:UNDECYL-MALTOSIDE'>UMQ</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bl2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bl2 OCA], [https://pdbe.org/2bl2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bl2 RCSB], [https://www.ebi.ac.uk/pdbsum/2bl2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bl2 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NTPK_ENTHA NTPK_ENTHA] Involved in ATP-driven sodium extrusion.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bl/2bl2_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bl2 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The membrane rotor ring from the vacuolar-type (V-type) sodium ion-pumping adenosine triphosphatase (Na+-ATPase) from Enterococcus hirae consists of 10 NtpK subunits, which are homologs of the 16-kilodalton and 8-kilodalton proteolipids found in other V-ATPases and in F1Fo- or F-ATPases, respectively. Each NtpK subunit has four transmembrane alpha helices, with a sodium ion bound between helices 2 and 4 at a site buried deeply in the membrane that includes the essential residue glutamate-139. This site is probably connected to the membrane surface by two half-channels in subunit NtpI, against which the ring rotates. Symmetry mismatch between the rotor and catalytic domains appears to be an intrinsic feature of both V- and F-ATPases.
-==About this Structure==
+Structure of the rotor of the V-Type Na+-ATPase from Enterococcus hirae.,Murata T, Yamato I, Kakinuma Y, Leslie AG, Walker JE Science. 2005 Apr 29;308(5722):654-9. Epub 2005 Mar 31. PMID:15802565<ref>PMID:15802565</ref>
-BL2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_hirae Enterococcus hirae] with NA, LHG and UMQ as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] Structure known Active Site: . Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BL2 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Structure of the rotor of the V-Type Na+-ATPase from Enterococcus hirae., Murata T, Yamato I, Kakinuma Y, Leslie AG, Walker JE, Science. 2005 Apr 29;308(5722):654-9. Epub 2005 Mar 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15802565 15802565]
+</div>
+<div class="pdbe-citations 2bl2" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[ATPase 3D structures|ATPase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Enterococcus hirae]]
-[[Category: H(+)-transporting two-sector ATPase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Kakinuma Y]]
-[[Category: Kakinuma, Y.]]
+[[Category: Leslie AGW]]
-[[Category: Leslie, A.G.W.]]
+[[Category: Murata T]]
-[[Category: Murata, T.]]
+[[Category: Walker JE]]
-[[Category: Walker, J.E.]]
+[[Category: Yamato I]]
-[[Category: Yamato, I.]]
-[[Category: LHG]]
-[[Category: NA]]
-[[Category: UMQ]]
-[[Category: hydrogen ion transport]]
-[[Category: hydrolase]]
-[[Category: k-ring]]
-[[Category: membrane rotor]]
-[[Category: sodium transporter]]
-[[Category: transmembrane]]
-[[Category: v-type atpase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 15:02:20 2007''