5em1: Difference between revisions

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-'''Unreleased structure'''
-The entry 5em1 is ON HOLD  until Paper Publication
+==Crystal structure of ragweed allergen Amb a 8==
+<StructureSection load='5em1' size='340' side='right'caption='[[5em1]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5em1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ambrosia_artemisiifolia Ambrosia artemisiifolia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EM1 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5em1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5em1 OCA], [https://pdbe.org/5em1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5em1 RCSB], [https://www.ebi.ac.uk/pdbsum/5em1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5em1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q2KN24_AMBAR Q2KN24_AMBAR] Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations.[RuleBase:RU003908]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Ragweed allergens affect several million people in the USA and Canada. To date, only two ragweed allergens, Amb t 5 and Amb a 11, have their structures determined and deposited to the Protein Data Bank. Here, we present structures of methylated ragweed allergen Amb a 8, Amb a 8 in the presence of poly-L-proline and Art v 4 (mugwort allergen). Amb a 8 and Art v 4 are panallergens belonging to the profilin family of proteins. They share significant sequence and structural similarities which results in cross-recognition by IgE antibodies. Molecular and immunological properties of Amb a 8 and Art v 4 are compared to those of Bet v 2 (birch pollen allergen), as well as to other allergenic profilins. We purified recombinant allergens that are recognized by patient IgE and are highly cross-reactive. It was determined that the analyzed allergens are relatively unstable. Structures of Amb a 8 in complex with poly-L-proline10 or poly-L-proline14 are the first structures of the plant profilin in complex with proline-rich peptides. Amb a 8 binds the poly-L-proline in a mode similar to that observed in human, mouse and P. falciparum profilin-peptide complexes. However, only some of residues that form the peptide binding site are conserved.
-Authors: Offermann, L.R., He, J.Z., Perdue, M.L., Chruszcz, M.
+Structural, functional and immunological characterization of profilin panallergens amb a 8, Art v 4, and Bet v 2.,Offermann LR, Schlachter CR, Perdue ML, Majorek KA, He JZ, Booth WT, Garrett J, Kowal K, Chruszcz M J Biol Chem. 2016 May 26. pii: jbc.M116.733659. PMID:27231348<ref>PMID:27231348</ref>
-Description: Crystal structure of ragweed allergen Amb a 8
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: He, J.Z]]
+<div class="pdbe-citations 5em1" style="background-color:#fffaf0;"></div>
-[[Category: Perdue, M.L]]
-[[Category: Chruszcz, M]]
+==See Also==
-[[Category: Offermann, L.R]]
+*[[Profilin 3D Structures|Profilin 3D Structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Ambrosia artemisiifolia]]
+[[Category: Large Structures]]
+[[Category: Chruszcz M]]
+[[Category: He JZ]]
+[[Category: Offermann LR]]
+[[Category: Perdue ML]]