Barnase: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Jaime Prilusky, Alexander Berchansky

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-<StructureSection load='1brn' size='450' side='right' scene= caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'>
+<StructureSection load='1brn' size='350' side='right' scene='44/442736/Cv/2' caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'>
 == Function ==
-'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''.  The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
+'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial [[ribonuclease]] synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
 == Relevance ==
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 == Structural highlights ==
-BA hydrolyzes RNA at GpN sites where the positively charged Lys and Arg coordinate the RNA phosphate group.
+BA hydrolyzes RNA at GpN sites where the <scene name='44/442736/Cv/4'>active site positively charged Lys and Arg coordinate the RNA phosphate group</scene> (Lys and Arg are in magenta).
 == 3D Structures of Barnase ==
+[[Barnase 3D structures]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+</StructureSection>
-{{#tree:id=OrganizedByTopic|openlevels=0|
-*Barnase
+== References ==
+<references/>
-**[[3kch]], [[2f4y]] – BA cross-linked<br />
-**[[2f56]] - BA cross-linked + urea<br />
-**[[2f5w]] - BA cross-linked + thiourea<br />
-**[[2f5m]] - BA cross-linked + Bromo-ethanol<br />
-**[[1fw7]], [[1bnr]] – BA - NMR<br />
-**[[2kf3]] – BA (mutant) – NMR<br />
-**[[2kf4]], [[1b2x]], [[1a2p]], [[1bni]], [[1bnj]] – BA<br />
-**[[1yvs]] – BA domain swapped<br />
-**[[1b20]], [[1b21]], [[1b2z]], [[1bne]], [[1bnf]], [[1bng]], [[1brh]], [[1bri]], [[1brj]], [[1brk]], [[1bns]], [[1brg]], [[1bsa]], [[1bsb]], [[1bsc]], [[1bsd]], [[1bse]], [[1ban]], [[1bao]] – BA (mutant)<br />
-**[[2kf5]], [[2kf6]], [[1brn]], [[1rnb]] – BA + polynucleotide
-*Barnase + BST
-**[[3da7]] - BA (mutant) + BST<br />
-**[[2za4]], [[1brs]], [[1bgs]] – BA + BST<br />
-**[[1x1u]], [[1x1w]], [[1x1x]], [[1x1y]], [[1b27]], [[1b2u]] – BA + BST (mutant)<br />
-**[[1b3s]], [[1b2s]] - BA (mutant) + BST (mutant)<br />
-**[[2c4b]] – BA/MCOEETI (mutant)
-}}
 [[Category:Topic Page]]