1gvy: Difference between revisions

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-[[Image:1gvy.gif|left|200px]]<br />
-<applet load="1gvy" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1gvy, resolution 1.70&Aring;" />
-'''SUBSTRATE DISTORSION BY BETA-MANNANASE FROM PSEUDOMONAS CELLULOSA'''<br />
-==About this Structure==
+==Substrate distorsion by beta-mannanase from Pseudomonas cellulosa==
-GVY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus] with ZN, NA, NIN, MBF and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] Structure known Active Site: NIN. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVY OCA].
+<StructureSection load='1gvy' size='340' side='right'caption='[[1gvy]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1gvy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GVY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GVY FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MBF:2-DEOXY-2-FLUORO-BETA-D-MANNOSE'>MBF</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NIN:DINITROPHENYLENE'>NIN</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gvy OCA], [https://pdbe.org/1gvy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gvy RCSB], [https://www.ebi.ac.uk/pdbsum/1gvy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gvy ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MANA_CELJU MANA_CELJU] Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides.<ref>PMID:7848261</ref> <ref>PMID:11064195</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gv/1gvy_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gvy ConSurf].
+<div style="clear:both"></div>
-==Reference==
+==See Also==
-Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state., Ducros VM, Zechel DL, Murshudov GN, Gilbert HJ, Szabo L, Stoll D, Withers SG, Davies GJ, Angew Chem Int Ed Engl. 2002 Aug 2;41(15):2824-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12203498 12203498]
+*[[Mannosidase 3D structures|Mannosidase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Cellvibrio japonicus]]
-[[Category: Mannan endo-1,4-beta-mannosidase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Davies GJ]]
-[[Category: Davies, G.J.]]
+[[Category: Ducros V]]
-[[Category: Ducros, V.]]
+[[Category: Gilbert HJ]]
-[[Category: Gilbert, H.J.]]
+[[Category: Szabo L]]
-[[Category: Stoll, D.]]
+[[Category: Withers SG]]
-[[Category: Szabo, L.]]
+[[Category: Zechel DL]]
-[[Category: Withers, S.G.]]
-[[Category: Zechel, D.L.]]
-[[Category: MBF]]
-[[Category: NA]]
-[[Category: NIN]]
-[[Category: TRS]]
-[[Category: ZN]]
-[[Category: family 26]]
-[[Category: glycosidase]]
-[[Category: glycoside hydrolase]]
-[[Category: mannan]]
-[[Category: mannanase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 14:44:43 2007''