2hw0: Difference between revisions

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OCA

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 ==NMR Solution Structure of the nuclease domain from the Replicator Initiator Protein from porcine circovirus PCV2==
-<StructureSection load='2hw0' size='340' side='right' caption='[[2hw0]], [[NMR_Ensembles_of_Models | 31 NMR models]]' scene=''>
+<StructureSection load='2hw0' size='340' side='right'caption='[[2hw0]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2hw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pcv2 Pcv2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HW0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2hw0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_circovirus_2 Porcine circovirus 2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HW0 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rep, ORF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85708 PCV2])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hw0 OCA], [http://pdbe.org/2hw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hw0 RCSB], [http://www.ebi.ac.uk/pdbsum/2hw0 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hw0 OCA], [https://pdbe.org/2hw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2hw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hw0 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/REP_PCV2 REP_PCV2] Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, and nucleotidyl transferase.<ref>PMID:12954212</ref> <ref>PMID:25768890</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
    <jmolCheckbox>
-     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/2hw0_consurf.spt"</scriptWhenChecked>
+     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/2hw0_consurf.spt"</scriptWhenChecked>
      <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
    </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hw0 ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 29: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Pcv2]]
+[[Category: Large Structures]]
-[[Category: Byeon, I L]]
+[[Category: Porcine circovirus 2]]
-[[Category: Campos-Olivas, R]]
+[[Category: Byeon IL]]
-[[Category: Gronenborn, A M]]
+[[Category: Campos-Olivas R]]
-[[Category: Gronenborn, B]]
+[[Category: Gronenborn AM]]
-[[Category: Vega-Rocha, S]]
+[[Category: Gronenborn B]]
-[[Category: Alpha+beta]]
+[[Category: Vega-Rocha S]]
-[[Category: Hydrolase]]
-[[Category: Replication]]