2cg2: Difference between revisions

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-==CRYSTAL STRUCTURE OF SDSA1, AN ALKYLSULFATASE FROM PSEUDOMONAS AERUGINOSA, IN COMPLEX WITH SULFATE==
-<StructureSection load='2cg2' size='340' side='right' caption='[[2cg2]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+==Crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, in complex with sulfate==
+<StructureSection load='2cg2' size='340' side='right'caption='[[2cg2]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2cg2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CG2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CG2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2cg2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CG2 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2cfu|2cfu]], [[2cfz|2cfz]], [[2cg3|2cg3]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cg2 OCA], [http://pdbe.org/2cg2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cg2 RCSB], [http://www.ebi.ac.uk/pdbsum/2cg2 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cg2 OCA], [https://pdbe.org/2cg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cg2 RCSB], [https://www.ebi.ac.uk/pdbsum/2cg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cg2 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/LPAKS_PSEAE LPAKS_PSEAE] Alkylsulfatase that cleaves both short and medium-length alkylsulfates, including the widely used detergent sodium dodecyl sulfate (SDS) (PubMed:16684886). Acts in vitro on primary alkylsulfates such as decyl sulfate, octyl sulfate and hexyl sulfate, but not on sulfated sugars and arylsulfates (PubMed:16684886, PubMed:23061549). The hydrolysis of SDS allows the bacterium to use SDS as a sole carbon or sulfur source (PubMed:16684886). In addition, contributes to the in vivo virulence of P.aeruginosa by playing a central role in the degradation of mucin, a glycoprotein that is the primary component of the mucus overlaying the host epithelial tissues (PubMed:31907968). Plays a role in the degradation of both protein and carbohydrate moieties in mucin via the release of sulfate from mucin, D-galactose 6-sulfate (Gal-6S) and N-acetyl-D-glucosamine 6-sulfate (GlcNAc-6S) (PubMed:31907968).<ref>PMID:16684886</ref> <ref>PMID:23061549</ref> <ref>PMID:31907968</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
    <jmolCheckbox>
-     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/2cg2_consurf.spt"</scriptWhenChecked>
+     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/2cg2_consurf.spt"</scriptWhenChecked>
      <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
    </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cg2 ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 30: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Pseae]]
+[[Category: Large Structures]]
-[[Category: Adams, T M]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-[[Category: Hagelueken, G]]
+[[Category: Adams TM]]
-[[Category: Heinz, D W]]
+[[Category: Hagelueken G]]
-[[Category: Kolmar, H]]
+[[Category: Heinz DW]]
-[[Category: Schubert, W D]]
+[[Category: Kolmar H]]
-[[Category: Tuemmler, B]]
+[[Category: Schubert W-D]]
-[[Category: Widow, U]]
+[[Category: Tuemmler B]]
-[[Category: Wiehlmann, L]]
+[[Category: Widow U]]
-[[Category: Hydrolase]]
+[[Category: Wiehlmann L]]
-[[Category: Pseudomonas aeruginosa]]
-[[Category: Sds-hydrolase]]
-[[Category: Sdsa1]]