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[[Image:1fa2.gif|left|200px]]


{{Structure
==CRYSTAL STRUCTURE OF BETA-AMYLASE FROM SWEET POTATO==
|PDB= 1fa2 |SIZE=350|CAPTION= <scene name='initialview01'>1fa2</scene>, resolution 2.30&Aring;
<StructureSection load='1fa2' size='340' side='right'caption='[[1fa2]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=DOM:2&#39;-DEOXYMALTOSE'>DOM</scene> and <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>
<table><tr><td colspan='2'>[[1fa2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ipomoea_batatas Ipomoea batatas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FA2 FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-amylase Beta-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.2 3.2.1.2]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900048:2-deoxy-maltose'>PRD_900048</scene>, <scene name='pdbligand=RR7:2-deoxy-beta-D-arabino-hexopyranose'>RR7</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fa2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fa2 OCA], [https://pdbe.org/1fa2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fa2 RCSB], [https://www.ebi.ac.uk/pdbsum/1fa2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fa2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AMYB_IPOBA AMYB_IPOBA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fa/1fa2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fa2 ConSurf].
<div style="clear:both"></div>


'''CRYSTAL STRUCTURE OF BETA-AMYLASE FROM SWEET POTATO'''
==See Also==
 
*[[Amylase 3D structures|Amylase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Sweet potato beta-amylase is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry. Its subunit consists of 498 amino acid residues (Mr 55,880). It has been crystallized at room temperature using polyethylene glycol 1500 as precipitant. The crystals, growing to dimensions of 0.4 mm x 0.4 mm x 1.0 mm within 2 weeks, belong to the tetragonal space group P4(2)2(1)2 with unit cell dimensions of a = b = 129.63 A and c = 68.42 A. The asymmetric unit contains 1 subunit of beta-amylase, with a crystal volume per protein mass (VM) of 2.57 A3/Da and a solvent content of 52% by volume. The three-dimensional structure of the tetrameric beta-amylase from sweet potato has been determined by molecular replacement methods using the monomeric structure of soybean enzyme as the starting model. The refined subunit model contains 3,863 nonhydrogen protein atoms (488 amino acid residues) and 319 water oxygen atoms. The current R-value is 20.3% for data in the resolution range of 8-2.3 A (with 2 sigma cut-off) with good stereochemistry. The subunit structure of sweet potato beta-amylase (crystallized in the absence of alpha-cyclodextrin) is very similar to that of soybean beta-amylase (complexed with alpha-cyclodextrin). The root-mean-square (RMS) difference for 487 equivalent C alpha atoms of the two beta-amylases is 0.96 A. Each subunit of sweet potato beta-amylase is composed of a large (alpha/beta)8 core domain, a small one made up of three long loops [L3 (residues 91-150), L4 (residues 183-258), and L5 (residues 300-327)], and a long C-terminal loop formed by residues 445-493. Conserved Glu 187, believed to play an important role in catalysis, is located at the cleft between the (alpha/beta)8 barrel core and a small domain made up of three long loops (L3, L4, and L5). Conserved Cys 96, important in the inactivation of enzyme activity by sulfhydryl reagents, is located at the entrance of the (alpha/beta)8 barrel.
 
==About this Structure==
1FA2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ipomoea_batatas Ipomoea batatas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FA2 OCA].
 
==Reference==
Crystallization, molecular replacement solution, and refinement of tetrameric beta-amylase from sweet potato., Cheong CG, Eom SH, Chang C, Shin DH, Song HK, Min K, Moon JH, Kim KK, Hwang KY, Suh SW, Proteins. 1995 Feb;21(2):105-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7777485 7777485]
[[Category: Beta-amylase]]
[[Category: Ipomoea batatas]]
[[Category: Ipomoea batatas]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cheong, C G.]]
[[Category: Cheong CG]]
[[Category: Lee, B I.]]
[[Category: Lee BI]]
[[Category: Suh, S W.]]
[[Category: Suh SW]]
[[Category: DOM]]
[[Category: DTT]]
[[Category: tim barrel]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 11:45:39 2008''

Latest revision as of 10:12, 7 February 2024

CRYSTAL STRUCTURE OF BETA-AMYLASE FROM SWEET POTATOCRYSTAL STRUCTURE OF BETA-AMYLASE FROM SWEET POTATO

Structural highlights

1fa2 is a 1 chain structure with sequence from Ipomoea batatas. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AMYB_IPOBA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1fa2, resolution 2.30Å

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