1dtp: Difference between revisions

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-[[Image:1dtp.gif|left|200px]]
- {{Structure
+==THE STRUCTURE OF THE ISOLATED CATALYTIC DOMAIN OF DIPHTHERIA TOXIN==
-|PDB= 1dtp |SIZE=350|CAPTION= <scene name='initialview01'>1dtp</scene>, resolution 2.5&Aring;
+<StructureSection load='1dtp' size='340' side='right'caption='[[1dtp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=APU:ADENYLYL-3&#39;-5&#39;-PHOSPHO-URIDINE-3&#39;-MONOPHOSPHATE'>APU</scene>
+<table><tr><td colspan='2'>[[1dtp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynephage_beta Corynephage beta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DTP FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APU:ADENYLYL-3-5-PHOSPHO-URIDINE-3-MONOPHOSPHATE'>APU</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dtp OCA], [https://pdbe.org/1dtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dtp RCSB], [https://www.ebi.ac.uk/pdbsum/1dtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dtp ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DTX_CORBE DTX_CORBE] Diphtheria toxin, produced by a phage infecting Corynebacterium diphtheriae, is a proenzyme that, after activation, catalyzes the covalent attachment of the ADP ribose moiety of NAD to eukaryotic elongation factor 2 (eEF-2). Fragment A is the catalytic portion responsible for enzymatic ADP-ribosylation of elongation factor 2, while fragment B is responsible for binding of toxin to cell receptors and entry of fragment A.<ref>PMID:18276581</ref> <ref>PMID:19793133</ref>
-'''THE STRUCTURE OF THE ISOLATED CATALYTIC DOMAIN OF DIPHTHERIA TOXIN'''
+==See Also==
+*[[Diphtheria toxin|Diphtheria toxin]]
+== References ==
-==Overview==
+<references/>
-The structure of the isolated catalytic domain of diphtheria toxin at pH 5.0 was determined by X-ray crystallography at 2.5 A resolution and refined to an R-factor of 19.7%. The domain is bound to its endogenous inhibitor adenylyl(3'--&gt;5')uridine 3'-monophosphate (ApUp). The structure of this 190-residue domain, which was expressed in and isolated from Escherichia coli, is essentially identical to the structure of the catalytic domain within whole diphtheria toxin determined at pH 7.5. However, there are two adjacent surface loops (loop 66-78 and loop 169-176) that exhibit clear differences when compared to the structure of the catalytic domain in whole diphtheria toxin. Although both loops are at the surface of the protein and are relatively flexible, the chain trace is well-defined in the electron density. The main structural difference is the closer approach of loops 66-78 and 169-176. We ascribe this structural change mainly to the absence of the neighboring transmembrane domain in the isolated catalytic domain as compared to whole diphtheria toxin. We suggest that this change represents the first step of the structural transition from the catalytic domain in whole diphtheria toxin to the translocated form of the domain. The changes are described in detail, and their implications for membrane translocation are discussed.
+__TOC__
+</StructureSection>
-==About this Structure==
-DTP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Corynephage_beta Corynephage beta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DTP OCA].
-==Reference==
-Structure of the isolated catalytic domain of diphtheria toxin., Weiss MS, Blanke SR, Collier RJ, Eisenberg D, Biochemistry. 1995 Jan 24;34(3):773-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7827036 7827036]
 [[Category: Corynephage beta]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Eisenberg, D.]]
+[[Category: Eisenberg D]]
-[[Category: Weiss, M S.]]
+[[Category: Weiss MS]]
-[[Category: APU]]
-[[Category: toxin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 11:33:33 2008''