4ttu: Difference between revisions
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==N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with isomaltotriose== | ==N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with isomaltotriose== | ||
<StructureSection load='4ttu' size='340' side='right' caption='[[4ttu]], [[Resolution|resolution]] 2.18Å' scene=''> | <StructureSection load='4ttu' size='340' side='right'caption='[[4ttu]], [[Resolution|resolution]] 2.18Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ttu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TTU OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4ttu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leuconostoc_mesenteroides Leuconostoc mesenteroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TTU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TTU FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.18Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=IYR:3-IODO-TYROSINE'>IYR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TYI:3,5-DIIODOTYROSINE'>TYI</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ttu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ttu OCA], [https://pdbe.org/4ttu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ttu RCSB], [https://www.ebi.ac.uk/pdbsum/4ttu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ttu ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8G9Q2_LEUME Q8G9Q2_LEUME] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The alpha-(1-->2) branching sucrase DeltaN123-GBD-CD2 is an enzyme belonging to the Glycoside-Hydrolase family 70 (GH70) that catalyses the transfer of D-glucosyl units from sucrose to dextrans or glucooligosaccharides via the formation of alpha-(1-->2) glucosidic linkages. The first structures of DeltaN123-GBD-CD2 in complex with D-glucose, isomaltosyl or isomaltotriosyl residues were solved. The glucose complex revealed three glucose binding sites in the catalytic gorge and six additional ones at the surface of domains B, IV and V. Soaking with isomaltotriose or glucooligosaccharides led to structures in which isomaltosyl or isomaltriosyl residues were found in glucan binding pockets belonging to domain V. One aromatic residue is systematically identified at the bottom of these pockets in stacking interaction with one glucosyl moiety. The carbohydrate is also maintained by a network of hydrogen bonds and van der Waals interactions. The sequence of these binding pockets is conserved and repeatedly present in the domain V of GH70 glucansucrases known to bind alpha-glucans. These findings provide the first structural evidence of the molecular interaction occurring between isomaltooligosaccharides and the domain V of the GH70 enzymes. | |||
Structural insights into the carbohydrate-binding ability of an alpha-(1-->2) branching sucrase from glycoside-hydrolase family 70.,Brison Y, Malbert Y, Czaplicki G, Mourey L, Remaud-Simeon M, Tranier S J Biol Chem. 2016 Feb 10. pii: jbc.M115.688796. PMID:26865636<ref>PMID:26865636</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4ttu" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Brison | [[Category: Leuconostoc mesenteroides]] | ||
[[Category: Mourey | [[Category: Brison Y]] | ||
[[Category: Remaud-Simeon | [[Category: Mourey L]] | ||
[[Category: Tranier | [[Category: Remaud-Simeon M]] | ||
[[Category: Tranier S]] | |||