5ci6: Difference between revisions

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-'''Unreleased structure'''
-The entry 5ci6 is ON HOLD
+==Crystal structure of Arabidopsis thaliana MPK6==
+<StructureSection load='5ci6' size='340' side='right'caption='[[5ci6]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5ci6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CI6 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ci6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ci6 OCA], [https://pdbe.org/5ci6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ci6 RCSB], [https://www.ebi.ac.uk/pdbsum/5ci6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ci6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MPK6_ARATH MPK6_ARATH] Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking. May phosphorylate regulators of WRKY transcription factors. Phosphorylates 1-aminocyclopropane-1-carboxylic acid synthases (ACS2 and ACS6) and may be involved in the regulation of bacterial elicitor flagellin-induced ethylene production. Regulates locally gene-mediated and basal resistance response to certain pathogens. May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MKK1/MKK2 and MPK4/MPK6). MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H(2)O(2) production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination. MKK3-MPK6 module plays an important role in the jasmonate signal transduction pathway through the negative regulation of MYC2/JIN1 expression. MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection. MKK9-MPK6 module positively regulates leaf senescence. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs.<ref>PMID:11875555</ref> <ref>PMID:15020743</ref> <ref>PMID:15225555</ref> <ref>PMID:15539472</ref> <ref>PMID:15964670</ref> <ref>PMID:17259259</ref> <ref>PMID:17369371</ref> <ref>PMID:18248592</ref> <ref>PMID:18273012</ref> <ref>PMID:18378893</ref> <ref>PMID:19251906</ref> <ref>PMID:19484493</ref> <ref>PMID:23263767</ref> <ref>PMID:25010425</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Mitogen-activated protein kinase (MAPK) cascades, which are the highly conserved signalling modules in eukaryotic organisms, have been shown to play important roles in regulating growth, development, and stress responses. The structures of various MAPKs from yeast and animal have been solved, and structure-based mutants were generated for their function analyses, however, the structures of plant MAPKs remain unsolved. Here, we report the crystal structure of Arabidopsis MPK6 at a 3.0 A resolution. Although MPK6 is topologically similar to ERK2 and p38, the structures of the glycine-rich loop, MAPK insert, substrate binding sites, and L16 loop in MPK6 show notable differences from those of ERK2 and p38. Based on the structural comparison, we constructed MPK6 mutants and analyzed their kinase activity both in vitro and in planta. MPK6(F364L) and MPK6(F368L) mutants, in which Phe364 and Phe368 in the L16 loop were changed to Leu, respectively, acquired higher intrinsic kinase activity and retained the normal MAPKK activation property. The expression of MPK6 mutants with basal activity is sufficient to induce camalexin biosynthesis; however, to induce ethylene and leaf senescence, the expression of MPK6 mutants with higher activity is required. The results suggest that these mutants can be used to analyze the specific biological functions of MPK6.
-Authors: Qin, X., Li, P., Chen, Z., Ren, D.
+Analysis of crystal structure of Arabidopsis MPK6 and generation of its mutants with higher activity.,Wang B, Qin X, Wu J, Deng H, Li Y, Yang H, Chen Z, Liu G, Ren D Sci Rep. 2016 May 10;6:25646. doi: 10.1038/srep25646. PMID:27160427<ref>PMID:27160427</ref>
-Description: Crystal structure of Arabidopsis thaliana MPK6
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Ren, D]]
+<div class="pdbe-citations 5ci6" style="background-color:#fffaf0;"></div>
-[[Category: Chen, Z]]
+== References ==
-[[Category: Li, P]]
+<references/>
-[[Category: Qin, X]]
+__TOC__
+</StructureSection>
+[[Category: Arabidopsis thaliana]]
+[[Category: Large Structures]]
+[[Category: Chen Z]]
+[[Category: Li P]]
+[[Category: Qin X]]
+[[Category: Ren D]]