3sli: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:3sli.gif|left|200px]]
- {{Structure
+==LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2,7-ANHYDRO-NEU5AC PREPARED BY SOAKING WITH 3'-SIALYLLACTOSE==
-|PDB= 3sli |SIZE=350|CAPTION= <scene name='initialview01'>3sli</scene>, resolution 1.8&Aring;
+<StructureSection load='3sli' size='340' side='right'caption='[[3sli]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=SKD:2-ACETYLAMINO-7-(1,2-DIHYDROXY-ETHYL)-3-HYDROXY-6,8-DIOXA-BICYCLO[3.2.1]OCTANE-5-CARBOXYLIC ACID'>SKD</scene>
+<table><tr><td colspan='2'>[[3sli]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SLI FirstGlance]. <br>
-|ACTIVITY= [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-|GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6405 Macrobdella decora])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SKD:2-ACETYLAMINO-7-(1,2-DIHYDROXY-ETHYL)-3-HYDROXY-6,8-DIOXA-BICYCLO[3.2.1]OCTANE-5-CARBOXYLIC+ACID'>SKD</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sli OCA], [https://pdbe.org/3sli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sli RCSB], [https://www.ebi.ac.uk/pdbsum/3sli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sli ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NANL_MACDE NANL_MACDE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sl/3sli_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3sli ConSurf].
+<div style="clear:both"></div>
-'''LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2,7-ANHYDRO-NEU5AC PREPARED BY SOAKING WITH 3'-SIALYLLACTOSE'''
+==See Also==
+*[[Neuraminidase 3D structures|Neuraminidase 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-Intramolecular trans-sialidase from leech (Macrobdella decora) is the first member of the sialidase superfamily found to exhibit strict specificity towards the cleavage of terminal Neu5Acalpha2--&gt;3Gal linkage in sialoglycoconjugates. Its release of 2,7-anhydro-Neu5Ac instead of Neu5Ac indicates that it catalyzes an intramolecular trans-sialosyl reaction. Crystal structures of its complexes with an inactive substrate analogue 2-propenyl-Neu5Ac, and with the product 2,7-anhydro-Neu5Ac, have been determined to 1.8 A resolution. The boat conformation of the pyranose observed in the complexes supports the proposed enzymatic mechanism that O7 of an axial 6-glycerol group attacks the positively charged C2 of the intermediate. A generalized mechanism is proposed for the sialidase superfamily.
+[[Category: Large Structures]]
-==About this Structure==
-SLI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SLI OCA].
-==Reference==
-The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism., Luo Y, Li SC, Li YT, Luo M, J Mol Biol. 1999 Jan 8;285(1):323-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9878409 9878409]
-[[Category: Exo-alpha-sialidase]]
 [[Category: Macrobdella decora]]
-[[Category: Single protein]]
+[[Category: Li SC]]
-[[Category: Li, S C.]]
+[[Category: Li YT]]
-[[Category: Li, Y T.]]
+[[Category: Luo M]]
-[[Category: Luo, M.]]
+[[Category: Luo Y]]
-[[Category: Luo, Y.]]
-[[Category: SKD]]
-[[Category: hydrolase]]
-[[Category: intramolecular trans-sialidase]]
-[[Category: neuraminidase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:07:15 2008''