4qi8: Difference between revisions

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-'''Unreleased structure'''
-The entry 4qi8 is ON HOLD  until Sep 02 2016
+==Lytic polysaccharide monooxygenase 9F from Neurospora crassa, NcLPMO9F==
+<StructureSection load='4qi8' size='340' side='right'caption='[[4qi8]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4qi8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QI8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QI8 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qi8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qi8 OCA], [https://pdbe.org/4qi8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qi8 RCSB], [https://www.ebi.ac.uk/pdbsum/4qi8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qi8 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LP9F_NEUCR LP9F_NEUCR] Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 oxidation products (PubMed:23102010, PubMed:31835532, PubMed:35080911). Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate (By similarity).[UniProtKB:Q7SHI8]<ref>PMID:23102010</ref> <ref>PMID:31835532</ref> <ref>PMID:35080911</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A new paradigm for cellulose depolymerization by fungi focuses on an oxidative mechanism involving cellobiose dehydrogenases (CDH) and copper-dependent lytic polysaccharide monooxygenases (LPMO); however, mechanistic studies have been hampered by the lack of structural information regarding CDH. CDH contains a haem-binding cytochrome (CYT) connected via a flexible linker to a flavin-dependent dehydrogenase (DH). Electrons are generated from cellobiose oxidation catalysed by DH and shuttled via CYT to LPMO. Here we present structural analyses that provide a comprehensive picture of CDH conformers, which govern the electron transfer between redox centres. Using structure-based site-directed mutagenesis, rapid kinetics analysis and molecular docking, we demonstrate that flavin-to-haem interdomain electron transfer (IET) is enabled by a haem propionate group and that rapid IET requires a closed CDH state in which the propionate is tightly enfolded by DH. Following haem reduction, CYT reduces LPMO to initiate oxygen activation at the copper centre and subsequent cellulose depolymerization.
-Authors: Tan, T.C., Gandini, R., Sygmund, C., Kittl, R., Haltrich, D., Ludwig, R., Hallberg, B.M., Divne, C.
+Structural basis for cellobiose dehydrogenase action during oxidative cellulose degradation.,Tan TC, Kracher D, Gandini R, Sygmund C, Kittl R, Haltrich D, Hallberg BM, Ludwig R, Divne C Nat Commun. 2015 Jul 7;6:7542. doi: 10.1038/ncomms8542. PMID:26151670<ref>PMID:26151670</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Haltrich, D]]
+<div class="pdbe-citations 4qi8" style="background-color:#fffaf0;"></div>
-[[Category: Divne, C]]
-[[Category: Gandini, R]]
+==See Also==
-[[Category: Hallberg, B.M]]
+*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
-[[Category: Ludwig, R]]
+== References ==
-[[Category: Sygmund, C]]
+<references/>
-[[Category: Tan, T.C]]
+__TOC__
-[[Category: Kittl, R]]
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Neurospora crassa]]
+[[Category: Divne C]]
+[[Category: Gandini R]]
+[[Category: Hallberg BM]]
+[[Category: Haltrich D]]
+[[Category: Kittl R]]
+[[Category: Ludwig R]]
+[[Category: Sygmund C]]
+[[Category: Tan TC]]