4z4a: Difference between revisions

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-'''Unreleased structure'''
-The entry 4z4a is ON HOLD
+==Avirulence protein 4 (Avr4) from Pseudocercospora fuligena==
+<StructureSection load='4z4a' size='340' side='right'caption='[[4z4a]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4z4a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudocercospora_fuligena Pseudocercospora fuligena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z4A FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z4a OCA], [https://pdbe.org/4z4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z4a RCSB], [https://www.ebi.ac.uk/pdbsum/4z4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z4a ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/M3AZE0_PSEFD M3AZE0_PSEFD]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Chitin is a key component of fungal cell walls and a potent inducer of innate immune responses. Consequently, fungi may secrete chitin-binding lectins, such as the Cf-Avr4 effector protein from the tomato pathogen Cladosporium fulvum, to shield chitin from host-derived chitinases during infection. Homologs of Cf-Avr4 are found throughout Dothideomycetes and despite their modest primary sequence identity, many are perceived by the cognate tomato immune receptor Cf-4. Here, we determined the X-ray crystal structure of Pf-Avr4 from the tomato pathogen Pseudocercospora fuligena, thus providing a three-dimensional model of an Avr4 effector protein. In addition, we explored structural, biochemical, and functional aspects of Pf-Avr4 and Cf-Avr4 to further define the biology of core effector proteins and outline a conceptual framework for their pleiotropic recognition by single immune receptors. We show that Cf-Avr4 and Pf-Avr4 share functional specificity in binding (GlcNAc)6 and providing protection against plant- and microbial-derived chitinases, suggesting a broader role beyond deregulation of host immunity. Furthermore, structure-guided site-directed mutagenesis indicated that residues in Pf-Avr4 important for binding chitin do not directly influence recognition by Cf-4, and further suggested that the property of recognition is structurally separated, or does not fully overlap with the virulence function of the effector.
-Authors: Hurlburt, N.K., Kohler, A.C., Fisher, A.J.
+Structural analysis of an Avr4 effector ortholog offers insight into chitin-binding and recognition by the Cf-4 receptor.,Kohler AC, Chen LH, Hurlburt N, Salvucci A, Schwessinger B, Fisher AJ, Stergiopoulos I Plant Cell. 2016 Jul 8. pii: tpc.00893.2015. PMID:27401545<ref>PMID:27401545</ref>
-Description: Avirulence protein 4 (Avr4) from Pseudocercospora fuligena
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Fisher, A.J]]
+<div class="pdbe-citations 4z4a" style="background-color:#fffaf0;"></div>
-[[Category: Kohler, A.C]]
+== References ==
-[[Category: Hurlburt, N.K]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Pseudocercospora fuligena]]
+[[Category: Fisher AJ]]
+[[Category: Hurlburt NK]]
+[[Category: Kohler AC]]