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==Crystal Structure of (3R)-hydroxyacyl-ACP dehydratase HadAB hetero-dimer from Mycobacterium smegmatis==
==Crystal Structure of (3R)-hydroxyacyl-ACP dehydratase HadAB hetero-dimer from Mycobacterium smegmatis==
<StructureSection load='4rv2' size='340' side='right' caption='[[4rv2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
<StructureSection load='4rv2' size='340' side='right'caption='[[4rv2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4rv2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RV2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RV2 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4rv2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RV2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RV2 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rv2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rv2 RCSB], [http://www.ebi.ac.uk/pdbsum/4rv2 PDBsum]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rv2 OCA], [https://pdbe.org/4rv2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rv2 RCSB], [https://www.ebi.ac.uk/pdbsum/4rv2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rv2 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0QS40_MYCS2 A0QS40_MYCS2]
Fatty acid biosynthesis type II in mycobacteria delivers the fatty acids required for mycolic acid synthesis. The pathway employs a unique maoC like beta-hydroxyacyl-ACP dehydratase HadAB or HadBC heterodimer in the third step of the elongation cycle. Here we report the crystal structure of the HadAB complex determined using a Pb-SIRAS method. Crystal structure aided with enzymatic study establishes the roles of HadA as a scaffolding component and HadB as a catalytic component together indispensable for the activity. The detailed structural analysis of HadAB in combination with MD simulation endorses the spatial orientation of the central hot-dog helix and the dynamic nature of its associated loop in regulation of substrate specificities in dehydratase/hydratase family enzymes.
 
Crystal structure of dehydratase component HadAB complex of mycobacterial FAS-II pathway.,Biswas R, Dutta A, Dutta D, Hazra D, Banerjee DR, Basak A, Das AK Biochem Biophys Res Commun. 2015 Mar 6;458(2):369-74. doi:, 10.1016/j.bbrc.2015.01.119. Epub 2015 Feb 3. PMID:25656575<ref>PMID:25656575</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Biswas, R]]
[[Category: Large Structures]]
[[Category: Das, A K]]
[[Category: Mycolicibacterium smegmatis MC2 155]]
[[Category: Dutta, D]]
[[Category: Biswas R]]
[[Category: Hazra, D]]
[[Category: Das AK]]
[[Category: Hotdog fold]]
[[Category: Dutta D]]
[[Category: Lyase]]
[[Category: Hazra D]]

Latest revision as of 15:56, 1 March 2024

Crystal Structure of (3R)-hydroxyacyl-ACP dehydratase HadAB hetero-dimer from Mycobacterium smegmatisCrystal Structure of (3R)-hydroxyacyl-ACP dehydratase HadAB hetero-dimer from Mycobacterium smegmatis

Structural highlights

4rv2 is a 2 chain structure with sequence from Mycolicibacterium smegmatis MC2 155. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.7Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0QS40_MYCS2

4rv2, resolution 2.70Å

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