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==Crystal Structure of the PcaV transcriptional regulator from Streptomyces coelicolor in complex with its natural ligand==
==Crystal Structure of the PcaV transcriptional regulator from Streptomyces coelicolor in complex with its natural ligand==
<StructureSection load='4fht' size='340' side='right' caption='[[4fht]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
<StructureSection load='4fht' size='340' side='right'caption='[[4fht]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4fht]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor_a3(2) Streptomyces coelicolor a3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FHT FirstGlance]. <br>
<table><tr><td colspan='2'>[[4fht]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FHT FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DHB:3,4-DIHYDROXYBENZOIC+ACID'>DHB</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4g9y|4g9y]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DHB:3,4-DIHYDROXYBENZOIC+ACID'>DHB</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCO6704 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 Streptomyces coelicolor A3(2)])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fht OCA], [https://pdbe.org/4fht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fht RCSB], [https://www.ebi.ac.uk/pdbsum/4fht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fht ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fht OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fht RCSB], [http://www.ebi.ac.uk/pdbsum/4fht PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q9XAM6_STRCO Q9XAM6_STRCO]
MarR family proteins constitute a group of &gt;12 000 transcriptional regulators encoded in bacterial and archaeal genomes that control gene expression in metabolism, stress responses, virulence and multi-drug resistance. There is much interest in defining the molecular mechanism by which ligand binding attenuates the DNA-binding activities of these proteins. Here, we describe how PcaV, a MarR family regulator in Streptomyces coelicolor, controls transcription of genes encoding beta-ketoadipate pathway enzymes through its interaction with the pathway substrate, protocatechuate. This transcriptional repressor is the only MarR protein known to regulate this essential pathway for aromatic catabolism. In in vitro assays, protocatechuate and other phenolic compounds disrupt the PcaV-DNA complex. We show that PcaV binds protocatechuate in a 1:1 stoichiometry with the highest affinity of any MarR family member. Moreover, we report structures of PcaV in its apo form and in complex with protocatechuate. We identify an arginine residue that is critical for ligand coordination and demonstrate that it is also required for binding DNA. We propose that interaction of ligand with this arginine residue dictates conformational changes that modulate DNA binding. Our results provide new insights into the molecular mechanism by which ligands attenuate DNA binding in this large family of transcription factors.
 
Study of PcaV from Streptomyces coelicolor yields new insights into ligand-responsive MarR family transcription factors.,Davis JR, Brown BL, Page R, Sello JK Nucleic Acids Res. 2013 Apr 1;41(6):3888-900. doi: 10.1093/nar/gkt009. Epub 2013 , Feb 8. PMID:23396446<ref>PMID:23396446</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[Response regulator 3D structure|Response regulator 3D structure]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Brown, B L]]
[[Category: Large Structures]]
[[Category: Davis, J R]]
[[Category: Brown BL]]
[[Category: Page, R]]
[[Category: Davis JR]]
[[Category: Sello, J K]]
[[Category: Page R]]
[[Category: Marr family]]
[[Category: Sello JK]]
[[Category: Protocatechuate binding]]
[[Category: Transcription]]
[[Category: Transcription factor]]
[[Category: Winged helix-turn-helix]]

Latest revision as of 18:25, 14 March 2024

Crystal Structure of the PcaV transcriptional regulator from Streptomyces coelicolor in complex with its natural ligandCrystal Structure of the PcaV transcriptional regulator from Streptomyces coelicolor in complex with its natural ligand

Structural highlights

4fht is a 2 chain structure with sequence from Streptomyces coelicolor A3(2). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.15Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9XAM6_STRCO

See Also

4fht, resolution 2.15Å

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