2oq9: Difference between revisions
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==Structure of the non-canonical Mcol5 of Hydra nematocysts== | |||
<StructureSection load='2oq9' size='340' side='right'caption='[[2oq9]]' scene=''> | |||
| | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2oq9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hydra_vulgaris Hydra vulgaris]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OQ9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oq9 OCA], [https://pdbe.org/2oq9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oq9 RCSB], [https://www.ebi.ac.uk/pdbsum/2oq9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oq9 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A6N8P1_HYDVU A6N8P1_HYDVU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Minicollagens constitute a family of unusually short collagen molecules isolated from cnidarians. They are restricted to the nematocyst, a cylindrical explosive organelle serving in defense and capture of prey. The nematocyst capsule contains a long tubule inside of its matrix, which is expelled and everted during an ultrafast discharge process. Here, we report the cloning and characterization of a novel minicollagen in Hydra, designated minicollagen-15 (NCol-15). NCol-15, like NCol-3 and NCol-4, shows deviations from the canonical cysteine pattern in its terminal cysteine-rich domains (CRDs). Minicollagens share common domain architectures with a central collagen sequence flanked by polyproline stretches and short N- and C-terminal CRDs. The CRDs are involved in the formation of a highly resistant cysteine network, which constitutes the basic structure of the nematocyst capsule. Unlike NCol-1, which is part of the capsule wall, NCol-15 is localized to tubules, arguing for a functional differentiation of minicollagens within the nematocyst architecture. NMR analysis of the altered C-terminal CRD of NCol-15 showed a novel disulfide-linked structure within the cysteine-containing region exhibiting similar folding kinetics and stability as the canonical CRDs. Our data provide evidence for evolutionary diversification among minicollagens, which probably facilitated alterations in the morphology of the nematocyst wall and tubule. | |||
Minicollagen-15, a novel minicollagen isolated from Hydra, forms tubule structures in nematocysts.,Adamczyk P, Meier S, Gross T, Hobmayer B, Grzesiek S, Bachinger HP, Holstein TW, Ozbek S J Mol Biol. 2008 Feb 29;376(4):1008-20. Epub 2007 Dec 5. PMID:18206162<ref>PMID:18206162</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2oq9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Hydra vulgaris]] | |||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Grzesiek S]] | ||
[[Category: | [[Category: Meier S]] | ||
[[Category: | [[Category: Oezbek S]] | ||
[[Category: | |||
Latest revision as of 04:17, 21 November 2024
Structure of the non-canonical Mcol5 of Hydra nematocystsStructure of the non-canonical Mcol5 of Hydra nematocysts
Structural highlights
FunctionPublication Abstract from PubMedMinicollagens constitute a family of unusually short collagen molecules isolated from cnidarians. They are restricted to the nematocyst, a cylindrical explosive organelle serving in defense and capture of prey. The nematocyst capsule contains a long tubule inside of its matrix, which is expelled and everted during an ultrafast discharge process. Here, we report the cloning and characterization of a novel minicollagen in Hydra, designated minicollagen-15 (NCol-15). NCol-15, like NCol-3 and NCol-4, shows deviations from the canonical cysteine pattern in its terminal cysteine-rich domains (CRDs). Minicollagens share common domain architectures with a central collagen sequence flanked by polyproline stretches and short N- and C-terminal CRDs. The CRDs are involved in the formation of a highly resistant cysteine network, which constitutes the basic structure of the nematocyst capsule. Unlike NCol-1, which is part of the capsule wall, NCol-15 is localized to tubules, arguing for a functional differentiation of minicollagens within the nematocyst architecture. NMR analysis of the altered C-terminal CRD of NCol-15 showed a novel disulfide-linked structure within the cysteine-containing region exhibiting similar folding kinetics and stability as the canonical CRDs. Our data provide evidence for evolutionary diversification among minicollagens, which probably facilitated alterations in the morphology of the nematocyst wall and tubule. Minicollagen-15, a novel minicollagen isolated from Hydra, forms tubule structures in nematocysts.,Adamczyk P, Meier S, Gross T, Hobmayer B, Grzesiek S, Bachinger HP, Holstein TW, Ozbek S J Mol Biol. 2008 Feb 29;376(4):1008-20. Epub 2007 Dec 5. PMID:18206162[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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