2lua: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
==Solution structure of CXC domain of MSL2==
==Solution structure of CXC domain of MSL2==
<StructureSection load='2lua' size='340' side='right' caption='[[2lua]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2lua' size='340' side='right'caption='[[2lua]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2lua]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LUA FirstGlance]. <br>
<table><tr><td colspan='2'>[[2lua]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LUA FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">msl-2, CG3241 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lua OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lua RCSB], [http://www.ebi.ac.uk/pdbsum/2lua PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lua OCA], [https://pdbe.org/2lua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lua RCSB], [https://www.ebi.ac.uk/pdbsum/2lua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lua ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/MSL2_DROME MSL2_DROME] The Msl proteins are essential for elevating transcription of the single X chromosome in the male (X chromosome dosage compensation). Msl-2 is required for translation and/or stability of msl-1 in males. In complex with msl-1, acts as an E3 ubiquitin ligase that promotes ubiquitination of histone H2B.<ref>PMID:21726816</ref> <ref>PMID:7588059</ref> <ref>PMID:7781064</ref> <ref>PMID:7796814</ref>  
The dosage compensation complex (DCC) binds to single X chromosomes in Drosophila males and increases the transcription level of X-linked genes by approximately twofold. Male-specific lethal 2 (MSL2) together with MSL1 mediates the initial recruitment of the DCC to high-affinity sites in the X chromosome. MSL2 contains a DNA-binding cysteine-rich CXC domain that is important for X targeting. In this study, we determined the solution structure of MSL2 CXC domain by NMR spectroscopy. We identified three zinc ions in the CXC domain and determined the metal-to-cysteine connectivities from (1)H-(113)Cd correlation experiments. The structure reveals an unusual zinc-cysteine cluster composed of three zinc ions coordinated by six terminal and three bridging cysteines. The CXC domain exhibits unexpected structural homology to pre-SET motifs of histone lysine methyltransferases, expanding the distribution and structural diversity of the CXC domain superfamily. Our findings provide novel structural insight into the evolution and function of CXC domains.
 
Solution Structure of MSL2 CXC Domain Reveals an Unusual Zn(3)Cys(9) Cluster and Similarity to Pre-SET Domains of Histone Lysine Methyltransferases.,Zheng S, Wang J, Feng Y, Wang J, Ye K PLoS One. 2012;7(9):e45437. doi: 10.1371/journal.pone.0045437. Epub 2012 Sep 20. PMID:23029009<ref>PMID:23029009</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
== References ==
<references/>
<references/>
Line 20: Line 15:
</StructureSection>
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Feng, Y]]
[[Category: Large Structures]]
[[Category: Wang, J]]
[[Category: Feng Y]]
[[Category: Ye, K]]
[[Category: Wang J]]
[[Category: Zheng, S]]
[[Category: Ye K]]
[[Category: Dna binding protein]]
[[Category: Zheng S]]
[[Category: Metal binding]]

Latest revision as of 09:57, 1 May 2024

Solution structure of CXC domain of MSL2Solution structure of CXC domain of MSL2

Structural highlights

2lua is a 1 chain structure with sequence from Drosophila melanogaster. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MSL2_DROME The Msl proteins are essential for elevating transcription of the single X chromosome in the male (X chromosome dosage compensation). Msl-2 is required for translation and/or stability of msl-1 in males. In complex with msl-1, acts as an E3 ubiquitin ligase that promotes ubiquitination of histone H2B.[1] [2] [3] [4]

References

  1. ↑ Wu L, Zee BM, Wang Y, Garcia BA, Dou Y. The RING finger protein MSL2 in the MOF complex is an E3 ubiquitin ligase for H2B K34 and is involved in crosstalk with H3 K4 and K79 methylation. Mol Cell. 2011 Jul 8;43(1):132-44. doi: 10.1016/j.molcel.2011.05.015. PMID:21726816 doi:http://dx.doi.org/10.1016/j.molcel.2011.05.015
  2. ↑ Bashaw GJ, Baker BS. The msl-2 dosage compensation gene of Drosophila encodes a putative DNA-binding protein whose expression is sex specifically regulated by Sex-lethal. Development. 1995 Oct;121(10):3245-58. PMID:7588059
  3. ↑ Kelley RL, Solovyeva I, Lyman LM, Richman R, Solovyev V, Kuroda MI. Expression of msl-2 causes assembly of dosage compensation regulators on the X chromosomes and female lethality in Drosophila. Cell. 1995 Jun 16;81(6):867-77. PMID:7781064
  4. ↑ Zhou S, Yang Y, Scott MJ, Pannuti A, Fehr KC, Eisen A, Koonin EV, Fouts DL, Wrightsman R, Manning JE, et al.. Male-specific lethal 2, a dosage compensation gene of Drosophila, undergoes sex-specific regulation and encodes a protein with a RING finger and a metallothionein-like cysteine cluster. EMBO J. 1995 Jun 15;14(12):2884-95. PMID:7796814
Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=2lua&oldid=4136398"