Sandbox Reserved 957: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA, Michael Pierrelee

@@ Line 7: / Line 7: @@
 There are 11 families of PDE (from 1 to 9), there is 21 genes for PDE which code 60 different PDE. For the PDE5A, the only PDE5 subcategory, there are 4 isoforms but their catalytic domain is the same <ref>Byung-Je Sung, Kwang Yeon Hwang, Young Ho Jeon, Jae Il Lee, Yong-Seok Heo, Jin Hwan Kim, Jinho Moon, Jung Min Yoon, Young-Lan Hyun, Eunmi Kim, Sung Jin Eum, Sam-Yong Park, Jie-Oh Lee, Tae Gyu Lee, Seonggu Ro & Joong Myung Cho, Structure of the catalytic domain of human phosphodiesterase 5 with bound drug molecules, NATURE, VOL 425, 4 SEPTEMBER 2003</ref>.<br \>
 The catalytic reaction is the hydrolysis of guanosine cyclic monophosphate into linear guanosine monophosphate. This cGMP-specific enzyme have 3 domains (from N terminal to C terminal) : GAF A, GAF B and a conserved catalytic domain regard to other PDEs of the family. Only cGMP can bind GAF A or GAF B and it stimulates the hydrolysis.<br \>
-We study here the PDE5A catalytic fragment formed of amino acid residues from the 535th to the 860th <ref>PDB</ref>. In the inhibition, we talk about the Sildenafil mostly, because it's the most known (active ingredient in the Viagra®).<br \>
+We study here the PDE5A catalytic fragment formed of amino acid residues from the 535th to the 860th <ref>http://www.rcsb.org/pdb/explore.do?structureId=2H40</ref>. In the inhibition, we talk about the Sildenafil mostly, because it's the most known (active ingredient in the Viagra®).<br \>
 Problem in the PBD files: N-loop (from the 788th to the 881th residues) is not complete.