Sandbox Reserved 958: Difference between revisions
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- ''in vivo'' it catalyzes the '''transport the viral DNA into the nucleus''', it interacts with many proteins ('''VPR, VBP1, HIV-matrix,...''') and DNA to form the '''Pre-Integration Complex''' ('''PIC''') and allow the integration of the viral DNA into host genome and this mechanism can be divided in two reactions, the '''3'-Processing''' and the '''Strand Transfer'''<ref name="Dells">PMID: 19091057</ref> | - ''in vivo'' it catalyzes the '''transport the viral DNA into the nucleus''', it interacts with many proteins ('''VPR, VBP1, HIV-matrix,...''') and DNA to form the '''Pre-Integration Complex''' ('''PIC''') and allow the integration of the viral DNA into host genome and this mechanism can be divided in two reactions, the '''3'-Processing''' and the '''Strand Transfer'''<ref name="Dells">PMID: 19091057</ref> | ||
[[Image:3108751 idr-4-065f1.png]] | [[Image:3108751 idr-4-065f1.png | thumb | upright=3]] | ||
- ''in vitro'' researchers proved that two more reactions can be catalyzed by the integrase, the '''disintegration''' of the viral DNA and also a possible '''DNA polymerase activity'''<ref name="Liao">PMID:21426159</ref> to repair mismatches during integration. | - ''in vitro'' researchers proved that two more reactions can be catalyzed by the integrase, the '''disintegration''' of the viral DNA and also a possible '''DNA polymerase activity'''<ref name="Liao">PMID:21426159</ref> to repair mismatches during integration. | ||
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Studies have prooved that 4 different kind of '''P'''ost '''T'''ranslationnal '''M'''odifications ('''PTMs''') affect the HIV-1 integrase : ubiquitination, SUMOylation, acetylation and phosphorylation. Furthermore, there are proteins that counteract or facilitate these PTMs implantation, for instance '''p300 and GCN5 can acetylate IN''' mostly on its C-ter domain on three different Lys residues (K264, K266 and K273). | Studies have prooved that 4 different kind of '''P'''ost '''T'''ranslationnal '''M'''odifications ('''PTMs''') affect the HIV-1 integrase : ubiquitination, SUMOylation, acetylation and phosphorylation. Furthermore, there are proteins that counteract or facilitate these PTMs implantation, for instance '''p300 and GCN5 can acetylate IN''' mostly on its C-ter domain on three different Lys residues (K264, K266 and K273). | ||
[[Image:1742-4690-7-18-7.jpg]] | [[Image:1742-4690-7-18-7.jpg | thumb | upright=3]] | ||
In contrary '''Ku70 reduces the ubiquitination level''' of the N-ter domain (K6, K11, K27, K29, K33, K48 and K63). Another study also found that IN contains '''three ψ-K-x-D/E motifs''', which can be SUMOylated at three Lys residues, K46,<scene name='60/604477/Sumoylation/1'>K136</scene> and K244 <ref name="Terreni">PMID:20226045</ref>. | In contrary '''Ku70 reduces the ubiquitination level''' of the N-ter domain (K6, K11, K27, K29, K33, K48 and K63). Another study also found that IN contains '''three ψ-K-x-D/E motifs''', which can be SUMOylated at three Lys residues, K46,<scene name='60/604477/Sumoylation/1'>K136</scene> and K244 <ref name="Terreni">PMID:20226045</ref>. | ||