Sandbox Reserved 975: Difference between revisions
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== SUMO-conjugating enzyme UBC9 EC:6.3.2. == | == SUMO-conjugating enzyme UBC9 EC:6.3.2. == | ||
<StructureSection load='3uin' size='340' side='right' caption='Structure of UBC9' scene=''> | |||
==Context== | ==Context== | ||
The SUMO-conjugating enzyme UBC9 is involved in ubiquitination of proteins. | The SUMO-conjugating enzyme UBC9 is involved in ubiquitination of proteins. | ||
The Ubiquitin is a small protein of 76 aa. | The Ubiquitin is a small protein of 76 aa. | ||
[[Image:Bst0370937a03.gif|500px]] | |||
Ubiquitination is a post translationnal modification where an ubiquitin is attached to a protein. This modification has several consequences, it can lead to the degradation of the protein via the proteasome, it can change the protein localization or it can alter the interaction of the protein with other factors, this is why this modification plays an important role in the control of many cellular processes. | Ubiquitination is a post translationnal modification where an ubiquitin is attached to a protein. This modification has several consequences, it can lead to the degradation of the protein via the proteasome, it can change the protein localization or it can alter the interaction of the protein with other factors, this is why this modification plays an important role in the control of many cellular processes. | ||
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There is a negative patch surrounding the active site that is conserved between all UBCs, it is probably important in the interaction with Ubiquitin and E1, the common substrates of all UBC9s. | There is a negative patch surrounding the active site that is conserved between all UBCs, it is probably important in the interaction with Ubiquitin and E1, the common substrates of all UBC9s. | ||
But there are many particularities in the surface electric potential of UBC9, that probably reflect the specificity for E3 and the protein substrate. The electrostatic dipole is more important for UBC9 than for others UBCs. The positive charged are located on the | But there are many particularities in the surface electric potential of UBC9, that probably reflect the specificity for E3 and the protein substrate. The electrostatic dipole is more important for UBC9 than for others UBCs. The positive charged are located on the back face of the molecule. One, for example, is located on the N-terminal region, it is composed of a segment of basic residues separated by nonpolar residues (13RKAWRK18). This <scene name='60/604494/Postive_patch/1'>positive patch is located close to the β-hairpin </scene>, the presence of those two specifities in the same region could be a sign that this site is responsible for the specificity of UBC9 for some particular E3 and protein substrates. | ||
</StructureSection> | </StructureSection> | ||