2i9e: Difference between revisions

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-[[Image:2i9e.gif|left|200px]]
- {{Structure
+==Structure of Triosephosphate Isomerase of Tenebrio molitor==
-|PDB= 2i9e |SIZE=350|CAPTION= <scene name='initialview01'>2i9e</scene>, resolution 2.0&Aring;
+<StructureSection load='2i9e' size='340' side='right'caption='[[2i9e]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
+<table><tr><td colspan='2'>[[2i9e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Tenebrio_molitor Tenebrio molitor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I9E FirstGlance]. <br>
-|ACTIVITY= [http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-|GENE= tpi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7067 Tenebrio molitor])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i9e OCA], [https://pdbe.org/2i9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i9e RCSB], [https://www.ebi.ac.uk/pdbsum/2i9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i9e ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8MPF2_TENMO Q8MPF2_TENMO]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i9/2i9e_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i9e ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A coleopteran triosephosphate isomerase (TIM) from Tenebrio molitor (yellow mealworm beetle) was recombinantly expressed in Escherichia coli and characterized with respect to thermal stability, kinetic parameters and oligomeric state. The enzyme was successfully crystallized and the structure determined by X-ray analysis to 2.0 A resolution. This is the first example of an invertebrate TIM. We compare structural features with known structures of TIMs from microorganisms, plants and vertebrates, and discuss the utility of the Tenebrio TIM sequence, together with several newly sequenced insect TIMs, for molecular phylogenetic analysis.
-'''Structure of Triosephosphate Isomerase of Tenebrio molitor'''
+A coleopteran triosephosphate isomerase: X-ray structure and phylogenetic impact of insect sequences.,Knobeloch D, Schmidt A, Scheerer P, Krauss N, Wessner H, Scholz Ch, Kuttner G, von Rintelen T, Wessel A, Hohne W Insect Mol Biol. 2010 Feb;19(1):35-48. Epub 2009 Oct 22. PMID:19849721<ref>PMID:19849721</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-I9E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Tenebrio_molitor Tenebrio molitor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I9E OCA].
+<div class="pdbe-citations 2i9e" style="background-color:#fffaf0;"></div>
-[[Category: Single protein]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Tenebrio molitor]]
-[[Category: Triose-phosphate isomerase]]
+[[Category: Hoehne W]]
-[[Category: Hoehne, W.]]
+[[Category: Krauss N]]
-[[Category: Krauss, N.]]
+[[Category: Scheerer P]]
-[[Category: Scheerer, P.]]
+[[Category: Schmidt A]]
-[[Category: Schmidt, A.]]
+[[Category: Wessner H]]
-[[Category: Wessner, H.]]
-[[Category: TRS]]
-[[Category: triosephosphate isomerase tenebrio molitor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:26:49 2008''