4ccw: Difference between revisions
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==Crystal structure of naproxen esterase (carboxylesterase NP) from Bacillus subtilis== | ==Crystal structure of naproxen esterase (carboxylesterase NP) from Bacillus subtilis== | ||
<StructureSection load='4ccw' size='340' side='right' caption='[[4ccw]], [[Resolution|resolution]] 1.75Å' scene=''> | <StructureSection load='4ccw' size='340' side='right'caption='[[4ccw]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ccw]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4ccw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CCW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CCW FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VKC:(2-HYDROXYETHOXY)ACETIC+ACID'>VKC</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ccw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ccw OCA], [https://pdbe.org/4ccw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ccw RCSB], [https://www.ebi.ac.uk/pdbsum/4ccw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ccw ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q59248_BACIU Q59248_BACIU] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4ccw" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[Carboxylesterase|Carboxylesterase]] | *[[Carboxylesterase 3D structures|Carboxylesterase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bacillus | [[Category: Bacillus subtilis]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Dijkstra | [[Category: Dijkstra BW]] | ||
[[Category: Godinho | [[Category: Godinho LF]] | ||
[[Category: Nardini | [[Category: Nardini M]] | ||
[[Category: Quax | [[Category: Quax WJ]] | ||
[[Category: Rozeboom | [[Category: Rozeboom HJ]] | ||
Latest revision as of 15:07, 20 December 2023
Crystal structure of naproxen esterase (carboxylesterase NP) from Bacillus subtilisCrystal structure of naproxen esterase (carboxylesterase NP) from Bacillus subtilis
Structural highlights
FunctionPublication Abstract from PubMedNaproxen esterase (NP) from Bacillus subtilis Thai I-8 is a carboxylesterase that catalyzes the enantioselective hydrolysis of naproxenmethylester to produce S-naproxen (E>200). It is a homolog of CesA (98% sequence identity) and CesB (64% identity), both produced by B. subtilis strain 168. CesB can be used for the enantioselective hydrolysis of 1,2-O-isopropylideneglycerol (solketal) esters (E>200 for IPG-caprylate). Crystal structures of NP and CesB, determined to a resolution of 1.75A and 2.04A, respectively, showed that both proteins have a canonical alpha/beta hydrolase fold with an extra N-terminal helix stabilizing the cap subdomain. The active site in both enzymes is located in a deep hydrophobic groove and includes the catalytic triad residues Ser130, His274, and Glu245. A product analog, presumably 2-(2-hydroxyethoxy)acetic acid, was bound in the NP active site. The enzymes have different enantioselectivities, which previously were shown to result from only a few amino acid substitutions in the cap domain. Modeling of a substrate in the active site of NP allowed explaining the different enantioselectivities. In addition, Ala156 may be a determinant of enantioselectivity as well, since its side chain appears to interfere with the binding of certain R-enantiomers in the active site of NP. However, the exchange route for substrate and product between the active site and the solvent is not obvious from the structures. Flexibility of the cap domain might facilitate such exchange. Interestingly, both carboxylesterases show higher structural similarity to meta-cleavage compound (MCP) hydrolases than to other alpha/beta hydrolase fold esterases. Crystal structures of two Bacillus carboxylesterases with different enantioselectivities.,Rozeboom HJ, Godinho LF, Nardini M, Quax WJ, Dijkstra BW Biochim Biophys Acta. 2014 Mar;1844(3):567-75. doi: 10.1016/j.bbapap.2014.01.003., Epub 2014 Jan 11. PMID:24418394[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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