4mrv: Difference between revisions
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==Structure of a bacterial Atm1-family ABC transporter== | ==Structure of a bacterial Atm1-family ABC transporter== | ||
<StructureSection load='4mrv' size='340' side='right' caption='[[4mrv]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='4mrv' size='340' side='right'caption='[[4mrv]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4mrv]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4mrv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Novosphingobium_aromaticivorans_DSM_12444 Novosphingobium aromaticivorans DSM 12444]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MRV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MRV FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HGD:MERCURY+BIS(L-GAMMA-GLUTAMYL-3-SULFIDO-L-ALANYLGLYCINE)'>HGD</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand= | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HGD:MERCURY+BIS(L-GAMMA-GLUTAMYL-3-SULFIDO-L-ALANYLGLYCINE)'>HGD</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mrv OCA], [https://pdbe.org/4mrv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mrv RCSB], [https://www.ebi.ac.uk/pdbsum/4mrv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mrv ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4mrv" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Lee | [[Category: Novosphingobium aromaticivorans DSM 12444]] | ||
[[Category: Lewinson | [[Category: Lee JY]] | ||
[[Category: Rees | [[Category: Lewinson O]] | ||
[[Category: Yang | [[Category: Rees DC]] | ||
[[Category: Zhitnitsky | [[Category: Yang JG]] | ||
[[Category: Zhitnitsky D]] | |||
Latest revision as of 06:16, 21 November 2024
Structure of a bacterial Atm1-family ABC transporterStructure of a bacterial Atm1-family ABC transporter
Structural highlights
Publication Abstract from PubMedAlthough substantial progress has been achieved in the structural analysis of exporters from the superfamily of adenosine triphosphate (ATP)-binding cassette (ABC) transporters, much less is known about how they selectively recognize substrates and how substrate binding is coupled to ATP hydrolysis. We have addressed these questions through crystallographic analysis of the Atm1/ABCB7/HMT1/ABCB6 ortholog from Novosphingobium aromaticivorans DSM 12444, NaAtm1, at 2.4 angstrom resolution. Consistent with a physiological role in cellular detoxification processes, functional studies showed that glutathione derivatives can serve as substrates for NaAtm1 and that its overexpression in Escherichia coli confers protection against silver and mercury toxicity. The glutathione binding site highlights the articulated design of ABC exporters, with ligands and nucleotides spanning structurally conserved elements to create adaptable interfaces accommodating conformational rearrangements during the transport cycle. Structural basis for heavy metal detoxification by an Atm1-type ABC exporter.,Lee JY, Yang JG, Zhitnitsky D, Lewinson O, Rees DC Science. 2014 Mar 7;343(6175):1133-6. doi: 10.1126/science.1246489. PMID:24604198[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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