4ckp: Difference between revisions
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==Structure of an N-terminal fragment of Leishmania SAS-6 that contains part of its coiled coil domain== | ==Structure of an N-terminal fragment of Leishmania SAS-6 that contains part of its coiled coil domain== | ||
<StructureSection load='4ckp' size='340' side='right' caption='[[4ckp]], [[Resolution|resolution]] 3.45Å' scene=''> | <StructureSection load='4ckp' size='340' side='right'caption='[[4ckp]], [[Resolution|resolution]] 3.45Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ckp]] is a 6 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4ckp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CKP FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.45Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ckp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ckp OCA], [https://pdbe.org/4ckp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ckp RCSB], [https://www.ebi.ac.uk/pdbsum/4ckp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ckp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/E9AFQ5_LEIMA E9AFQ5_LEIMA] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4ckp" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Art:Turning cartwheels|Art:Turning cartwheels]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Leishmania major]] | ||
[[Category: | [[Category: Van Breugel M]] | ||
Latest revision as of 15:11, 20 December 2023
Structure of an N-terminal fragment of Leishmania SAS-6 that contains part of its coiled coil domainStructure of an N-terminal fragment of Leishmania SAS-6 that contains part of its coiled coil domain
Structural highlights
FunctionPublication Abstract from PubMedCentrioles are cylindrical cell organelles with a ninefold symmetric peripheral microtubule array that is essential to template cilia and flagella. They are built around a central cartwheel assembly that is organized through homo-oligomerization of the centriolar protein SAS-6, but whether SAS-6 self-assembly can dictate cartwheel and thereby centriole symmetry is unclear. Here we show that Leishmania major SAS-6 crystallizes as a 9-fold symmetric cartwheel and provide the X-ray structure of this assembly at a resolution of 3.5 A. We furthermore demonstrate that oligomerization of Leishmania SAS-6 can be inhibited by a small molecule in vitro and provide indications for its binding site. Our results firmly establish that SAS-6 can impose cartwheel symmetry on its own and indicate how this process might occur mechanistically in vivo. Importantly, our data also provide a proof-of-principle that inhibition of SAS-6 oligomerization by small molecules is feasible. DOI: http://dx.doi.org/10.7554/eLife.01812.001. Structure of the SAS-6 cartwheel hub from Leishmania major.,van Breugel M, Wilcken R, McLaughlin SH, Rutherford TJ, Johnson CM Elife. 2014 Jan 1;3:e01812. doi: 10.7554/eLife.01812. PMID:24596152[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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