2gqs: Difference between revisions

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[[Image:2gqs.gif|left|200px]]


{{Structure
==SAICAR Synthetase Complexed with CAIR-Mg2+ and ADP==
|PDB= 2gqs |SIZE=350|CAPTION= <scene name='initialview01'>2gqs</scene>, resolution 2.050&Aring;
<StructureSection load='2gqs' size='340' side='right'caption='[[2gqs]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
|SITE=
== Structural highlights ==
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=C2R:5-AMINO-1-(5-O-PHOSPHONO-BETA-D-RIBOFURANOSYL)-1H-IMIDAZOLE-4-CARBOXYLIC+ACID'>C2R</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene>
<table><tr><td colspan='2'>[[2gqs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GQS FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphoribosylaminoimidazolesuccinocarboxamide_synthase Phosphoribosylaminoimidazolesuccinocarboxamide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.6 6.3.2.6]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
|GENE= purC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=C2R:5-AMINO-1-(5-O-PHOSPHONO-BETA-D-RIBOFURANOSYL)-1H-IMIDAZOLE-4-CARBOXYLIC+ACID'>C2R</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gqs OCA], [https://pdbe.org/2gqs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gqs RCSB], [https://www.ebi.ac.uk/pdbsum/2gqs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gqs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PUR7_ECOLI PUR7_ECOLI]  
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gq/2gqs_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gqs ConSurf].
<div style="clear:both"></div>


'''SAICAR Synthetase Complexed with CAIR-Mg2+ and ADP'''
==See Also==
 
*[[SAICAR synthetase|SAICAR synthetase]]
 
__TOC__
==Overview==
</StructureSection>
Phosphoribosylaminoimidazole-succinocarboxamide synthetase (SAICAR synthetase) converts 4-carboxy-5-aminoimidazole ribonucleotide (CAIR) to 4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide (SAICAR). The enzyme is a target of natural products that impair cell growth. Reported here are the crystal structures of the ADP and the ADP.CAIR complexes of SAICAR synthetase from Escherichia coli, the latter being the first instance of a CAIR-ligated SAICAR synthetase. ADP and CAIR bind to the active site in association with three Mg(2+), two of which coordinate the same oxygen atom of the 4-carboxyl group of CAIR; whereas, the third coordinates the alpha- and beta-phosphoryl groups of ADP. The ADP.CAIR complex is the basis for a transition state model of a phosphoryl transfer reaction involving CAIR and ATP, but also supports an alternative chemical pathway in which the nucleophilic attack of l-aspartate precedes the phosphoryl transfer reaction. The polypeptide fold for residues 204-221 of the E. coli structure differs significantly from those of the ligand-free SAICAR synthetase from Thermatoga maritima and the adenine nucleotide complexes of the synthetase from Saccharomyces cerevisiae. Conformational differences between the E. coli, T. maritima, and yeast synthetases suggest the possibility of selective inhibition of de novo purine nucleotide biosynthesis in microbial organisms.
 
==About this Structure==
2GQS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQS OCA].
 
==Reference==
Nucleotide complexes of Escherichia coli phosphoribosylaminoimidazole succinocarboxamide synthetase., Ginder ND, Binkowski DJ, Fromm HJ, Honzatko RB, J Biol Chem. 2006 Jul 28;281(30):20680-8. Epub 2006 May 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16687397 16687397]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Phosphoribosylaminoimidazolesuccinocarboxamide synthase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Ginder ND]]
[[Category: Ginder, N D.]]
[[Category: Honzatko RB]]
[[Category: Honzatko, R B.]]
[[Category: ADP]]
[[Category: C2R]]
[[Category: FMT]]
[[Category: MG]]
[[Category: ade1]]
[[Category: ade2]]
[[Category: magnesium coordination]]
[[Category: metal coordination]]
[[Category: nucleotide complex]]
[[Category: purc]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:07:56 2008''

Latest revision as of 12:29, 14 February 2024

SAICAR Synthetase Complexed with CAIR-Mg2+ and ADPSAICAR Synthetase Complexed with CAIR-Mg2+ and ADP

Structural highlights

2gqs is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.05Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PUR7_ECOLI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2gqs, resolution 2.05Å

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