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==Catalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-H==
==Catalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-H==
<StructureSection load='3ki3' size='340' side='right' caption='[[3ki3]], [[Resolution|resolution]] 1.27&Aring;' scene=''>
<StructureSection load='3ki3' size='340' side='right'caption='[[3ki3]], [[Resolution|resolution]] 1.27&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3ki3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KI3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KI3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3ki3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KI3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G9H:2-[(DIMETHYLAMINO)METHYL]-5,6-DIHYDROIMIDAZO[4,5,1-JK][1,4]BENZODIAZEPIN-7(4H)-ONE'>G9H</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.27&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ess|3ess]], [[2q6m|2q6m]], [[2q5t|2q5t]], [[3ki0|3ki0]], [[3ki1|3ki1]], [[3ki2|3ki2]], [[3ki4|3ki4]], [[3ki5|3ki5]], [[3ki6|3ki6]], [[3ki7|3ki7]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G9H:2-[(DIMETHYLAMINO)METHYL]-5,6-DIHYDROIMIDAZO[4,5,1-JK][1,4]BENZODIAZEPIN-7(4H)-ONE'>G9H</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">chxa, toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ki3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ki3 OCA], [https://pdbe.org/3ki3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ki3 RCSB], [https://www.ebi.ac.uk/pdbsum/3ki3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ki3 ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ki3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ki3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ki3 RCSB], [http://www.ebi.ac.uk/pdbsum/3ki3 PDBsum]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/Q5EK40_VIBCH Q5EK40_VIBCH]] An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.<ref>PMID:18276581</ref> <ref>PMID:19793133</ref>
[https://www.uniprot.org/uniprot/CHXA_VIBCL CHXA_VIBCL] An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.<ref>PMID:18276581</ref> <ref>PMID:19793133</ref>  


==See Also==
==See Also==
*[[Exotoxin|Exotoxin]]
*[[Exotoxin 3D structures|Exotoxin 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
[[Category: Edwards, P R]]
[[Category: Edwards PR]]
[[Category: Jorgensen, R]]
[[Category: Jorgensen R]]
[[Category: Merrill, A R]]
[[Category: Merrill AR]]
[[Category: Adp-ribosyl transferase]]
[[Category: Alpha-beta complex]]
[[Category: Diphthamide]]
[[Category: Toxin]]
[[Category: Transferase]]

Latest revision as of 11:16, 6 September 2023

Catalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-HCatalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-H

Structural highlights

3ki3 is a 1 chain structure with sequence from Vibrio cholerae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.27Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CHXA_VIBCL An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.[1] [2]

See Also

References

  1. ↑ Jorgensen R, Purdy AE, Fieldhouse RJ, Kimber MS, Bartlett DH, Rod Merrill A. Cholix toxin, a novel ADP-ribosylating factor from vibrio cholerae. J Biol Chem. 2008 Feb 25;. PMID:18276581 doi:M710008200
  2. ↑ Turgeon Z, White D, Jorgensen R, Visschedyk D, Fieldhouse RJ, Mangroo D, Merrill AR. Yeast as a tool for characterizing mono-ADP-ribosyltransferase toxins. FEMS Microbiol Lett. 2009 Nov;300(1):97-106. Epub 2009 Aug 31. PMID:19793133 doi:10.1111/j.1574-6968.2009.01777.x

3ki3, resolution 1.27Å

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