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==Crystal structure of a putative ssRNA endonuclease Cas2, CRISPR adaptation protein from E.coli==
==Crystal structure of a putative ssRNA endonuclease Cas2, CRISPR adaptation protein from E.coli==
<StructureSection load='4mak' size='340' side='right' caption='[[4mak]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
<StructureSection load='4mak' size='340' side='right'caption='[[4mak]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4mak]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_str._k-12_substr._mg1655 Escherichia coli str. k-12 substr. mg1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MAK FirstGlance]. <br>
<table><tr><td colspan='2'>[[4mak]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._MG1655 Escherichia coli str. K-12 substr. MG1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MAK FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ygbF, cas2, b2754, JW5438 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511145 Escherichia coli str. K-12 substr. MG1655])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mak RCSB], [http://www.ebi.ac.uk/pdbsum/4mak PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mak OCA], [https://pdbe.org/4mak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mak RCSB], [https://www.ebi.ac.uk/pdbsum/4mak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mak ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CAS2_ECOLI CAS2_ECOLI]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease (By similarity).<ref>PMID:21255106</ref>
[https://www.uniprot.org/uniprot/CAS2_ECOLI CAS2_ECOLI] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease (By similarity).<ref>PMID:21255106</ref>  


==See Also==
==See Also==
*[[Endonuclease|Endonuclease]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia coli str. k-12 substr. mg1655]]
[[Category: Escherichia coli str. K-12 substr. MG1655]]
[[Category: Brown, G]]
[[Category: Large Structures]]
[[Category: Joachimiak, A]]
[[Category: Brown G]]
[[Category: Structural genomic]]
[[Category: Joachimiak A]]
[[Category: Nocek, B]]
[[Category: Nocek B]]
[[Category: Skarina, T]]
[[Category: Skarina T]]
[[Category: Yakunin, A]]
[[Category: Yakunin A]]
[[Category: Cas2]]
[[Category: Crispr]]
[[Category: Hydrolase]]
[[Category: Mcsg]]
[[Category: Psi-biology]]

Latest revision as of 15:24, 1 March 2024

Crystal structure of a putative ssRNA endonuclease Cas2, CRISPR adaptation protein from E.coliCrystal structure of a putative ssRNA endonuclease Cas2, CRISPR adaptation protein from E.coli

Structural highlights

4mak is a 2 chain structure with sequence from Escherichia coli str. K-12 substr. MG1655. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.1Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAS2_ECOLI CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease (By similarity).[1]

See Also

References

  1. ↑ Perez-Rodriguez R, Haitjema C, Huang Q, Nam KH, Bernardis S, Ke A, DeLisa MP. Envelope stress is a trigger of CRISPR RNA-mediated DNA silencing in Escherichia coli. Mol Microbiol. 2011 Feb;79(3):584-99. doi: 10.1111/j.1365-2958.2010.07482.x. Epub, 2010 Dec 13. PMID:21255106 doi:10.1111/j.1365-2958.2010.07482.x

4mak, resolution 1.10Å

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