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==B. subtilis SsbB/ssDNA==
==B. subtilis SsbB/ssDNA==
<StructureSection load='3vdy' size='340' side='right' caption='[[3vdy]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='3vdy' size='340' side='right'caption='[[3vdy]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3vdy]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VDY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VDY FirstGlance]. <br>
<table><tr><td colspan='2'>[[3vdy]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VDY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VDY FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ssbB, ywpH, BSU36310 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vdy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vdy RCSB], [http://www.ebi.ac.uk/pdbsum/3vdy PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vdy OCA], [https://pdbe.org/3vdy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vdy RCSB], [https://www.ebi.ac.uk/pdbsum/3vdy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vdy ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/SSB2_BACSU SSB2_BACSU]] Not essential for replication of the chromosome but is required for optimal competence. Probably binds ssDNA and allows DNA recombination.<ref>PMID:14762004</ref> 
[https://www.uniprot.org/uniprot/SSBB_BACSU SSBB_BACSU] Not essential for replication of the chromosome but is required for optimal competence. Probably binds ssDNA and allows DNA recombination.<ref>PMID:14762004</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We have investigated the structural, biochemical and cellular roles of the two single-stranded (ss) DNA-binding proteins from Bacillus subtilis, SsbA and SsbB. During transformation, SsbB localizes at the DNA entry pole where it binds and protects internalized ssDNA. The 2.8-A resolution structure of SsbB bound to ssDNA reveals a similar overall protein architecture and ssDNA-binding surface to that of Escherichia coli SSB. SsbA, which binds ssDNA with higher affinity than SsbB, co-assembles onto SsbB-coated ssDNA and the two proteins inhibit ssDNA binding by the recombinase RecA. During chromosomal transformation, the RecA mediators RecO and DprA provide RecA access to ssDNA. Interestingly, RecO interaction with ssDNA-bound SsbA helps to dislodge both SsbA and SsbB from the DNA more efficiently than if the DNA is coated only with SsbA. Once RecA is nucleated onto the ssDNA, RecA filament elongation displaces SsbA and SsbB and enables RecA-mediated DNA strand exchange. During plasmid transformation, RecO localizes to the entry pole and catalyzes annealing of SsbA- or SsbA/SsbB-coated complementary ssDNAs to form duplex DNA with ssDNA tails. Our results provide a mechanistic framework for rationalizing the coordinated events modulated by SsbA, SsbB and RecO that are crucial for RecA-dependent chromosomal transformation and RecA-independent plasmid transformation.
 
Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins.,Yadav T, Carrasco B, Myers AR, George NP, Keck JL, Alonso JC Nucleic Acids Res. 2012 Feb 28. PMID:22373918<ref>PMID:22373918</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Alonso, J C]]
[[Category: Large Structures]]
[[Category: Carrasco, B]]
[[Category: Synthetic construct]]
[[Category: George, N P]]
[[Category: Alonso JC]]
[[Category: Keck, J L]]
[[Category: Carrasco B]]
[[Category: Myers, A R]]
[[Category: George NP]]
[[Category: Yadav, T]]
[[Category: Keck JL]]
[[Category: Dna binding protein-dna complex]]
[[Category: Myers AR]]
[[Category: Ob fold]]
[[Category: Yadav T]]
[[Category: Single-strand dna binding]]
[[Category: Ssdna]]

Latest revision as of 13:35, 1 March 2024

B. subtilis SsbB/ssDNAB. subtilis SsbB/ssDNA

Structural highlights

3vdy is a 7 chain structure with sequence from Bacillus subtilis and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SSBB_BACSU Not essential for replication of the chromosome but is required for optimal competence. Probably binds ssDNA and allows DNA recombination.[1]

See Also

References

  1. ↑ Lindner C, Nijland R, van Hartskamp M, Bron S, Hamoen LW, Kuipers OP. Differential expression of two paralogous genes of Bacillus subtilis encoding single-stranded DNA binding protein. J Bacteriol. 2004 Feb;186(4):1097-105. PMID:14762004

3vdy, resolution 2.80Å

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