1zs4: Difference between revisions

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[[Image:1zs4.gif|left|200px]]


{{Structure
==Structure of bacteriophage lambda cII protein in complex with DNA==
|PDB= 1zs4 |SIZE=350|CAPTION= <scene name='initialview01'>1zs4</scene>, resolution 1.7&Aring;
<StructureSection load='1zs4' size='340' side='right'caption='[[1zs4]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1zs4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZS4 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
|GENE= CII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zs4 OCA], [https://pdbe.org/1zs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zs4 RCSB], [https://www.ebi.ac.uk/pdbsum/1zs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zs4 ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''Structure of bacteriophage lambda cII protein in complex with DNA'''
[https://www.uniprot.org/uniprot/RPC2_LAMBD RPC2_LAMBD] This protein and protein CIII form a complex that is involved in the initiation of lysogeny. The complex binds at either of the cy regulatory sites preceding the CI repressor protein and integrase coding regions and induces transcription of these genes.
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
The tetrameric cII protein from bacteriophage lambda activates transcription from the phage promoters P(RE), P(I), and P(AQ) by binding to two direct repeats that flank the promoter -35 element. Here, we present the X-ray crystal structure of cII alone (2.8 A resolution) and in complex with its DNA operator from P(RE) (1.7 A resolution). The structures provide a basis for modeling of the activation complex with the RNA polymerase holoenzyme, and point to the key role for the RNA polymerase alpha subunit C-terminal domain (alphaCTD) in cII-dependent activation, which forms a bridge of protein/protein interactions between cII and the RNA polymerase sigma subunit. The model makes specific predictions for protein/protein interactions between cII and alphaCTD, and between alphaCTD and sigma, which are supported by previous genetic studies.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zs/1zs4_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
1ZS4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZS4 OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zs4 ConSurf].
Crystal structure of bacteriophage lambda cII and its DNA complex., Jain D, Kim Y, Maxwell KL, Beasley S, Zhang R, Gussin GN, Edwards AM, Darst SA, Mol Cell. 2005 Jul 22;19(2):259-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16039594 16039594]
<div style="clear:both"></div>
[[Category: Enterobacteria phage lambda]]
__TOC__
[[Category: Single protein]]
</StructureSection>
[[Category: Beasley, S.]]
[[Category: Escherichia virus Lambda]]
[[Category: Darst, S A.]]
[[Category: Large Structures]]
[[Category: Edwards, A M.]]
[[Category: Beasley S]]
[[Category: Gussin, G N.]]
[[Category: Darst SA]]
[[Category: Jain, D.]]
[[Category: Edwards AM]]
[[Category: Kim, Y.]]
[[Category: Gussin GN]]
[[Category: Maxwell, K L.]]
[[Category: Jain D]]
[[Category: helix-turn-helix]]
[[Category: Kim Y]]
[[Category: transcription activator]]
[[Category: Maxwell KL]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:39:12 2008''

Latest revision as of 12:06, 14 February 2024

Structure of bacteriophage lambda cII protein in complex with DNAStructure of bacteriophage lambda cII protein in complex with DNA

Structural highlights

1zs4 is a 6 chain structure with sequence from Escherichia virus Lambda. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RPC2_LAMBD This protein and protein CIII form a complex that is involved in the initiation of lysogeny. The complex binds at either of the cy regulatory sites preceding the CI repressor protein and integrase coding regions and induces transcription of these genes.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1zs4, resolution 1.70Å

Drag the structure with the mouse to rotate

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