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[[Image:1zc8.gif|left|200px]]


{{Structure
==Coordinates of tmRNA, SmpB, EF-Tu and h44 fitted into Cryo-EM map of the 70S ribosome and tmRNA complex==
|PDB= 1zc8 |SIZE=350|CAPTION= <scene name='initialview01'>1zc8</scene>
<SX load='1zc8' size='340' side='right' viewer='molstar' caption='[[1zc8]], [[Resolution|resolution]] 13.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1zc8]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZC8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZC8 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zc8 OCA], [https://pdbe.org/1zc8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zc8 RCSB], [https://www.ebi.ac.uk/pdbsum/1zc8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zc8 ProSAT]</span></td></tr>
}}
</table>
== Function ==
[https://www.uniprot.org/uniprot/SSRP_AQUAE SSRP_AQUAE] Binds specifically to the SsrA RNA (tmRNA) and is required for stable association of SsrA with ribosomes.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zc/1zc8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zc8 ConSurf].
<div style="clear:both"></div>


'''Coordinates of tmRNA, SmpB, EF-Tu and h44 fitted into Cryo-EM map of the 70S ribosome and tmRNA complex'''
==See Also==
 
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
 
__TOC__
==Overview==
</SX>
Bacterial ribosomes stalled on defective messenger RNAs (mRNAs) are rescued by tmRNA, an approximately 300-nucleotide-long molecule that functions as both transfer RNA (tRNA) and mRNA. Translation then switches from the defective message to a short open reading frame on tmRNA that tags the defective nascent peptide chain for degradation. However, the mechanism by which tmRNA can enter and move through the ribosome is unknown. We present a cryo-electron microscopy study at approximately 13 to 15 angstroms of the entry of tmRNA into the ribosome. The structure reveals how tmRNA could move through the ribosome despite its complicated topology and also suggests roles for proteins S1 and SmpB in the function of tmRNA.
[[Category: Large Structures]]
 
==About this Structure==
1ZC8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZC8 OCA].
 
==Reference==
Visualizing tmRNA entry into a stalled ribosome., Valle M, Gillet R, Kaur S, Henne A, Ramakrishnan V, Frank J, Science. 2003 Apr 4;300(5616):127-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12677067 12677067]
[[Category: Protein complex]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: Frank, J.]]
[[Category: Frank J]]
[[Category: Gillet, R.]]
[[Category: Gillet R]]
[[Category: Henne, A.]]
[[Category: Henne A]]
[[Category: Kaur, S.]]
[[Category: Kaur S]]
[[Category: Ramakrishnan, V.]]
[[Category: Ramakrishnan V]]
[[Category: Valle, M.]]
[[Category: Valle M]]
[[Category: 16s rrna]]
[[Category: 30]]
[[Category: 70]]
[[Category: cryo-em]]
[[Category: ef-tu]]
[[Category: h44]]
[[Category: smpb]]
[[Category: tmrna]]
[[Category: trans-translation]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:33:42 2008''

Latest revision as of 12:03, 14 February 2024

Coordinates of tmRNA, SmpB, EF-Tu and h44 fitted into Cryo-EM map of the 70S ribosome and tmRNA complexCoordinates of tmRNA, SmpB, EF-Tu and h44 fitted into Cryo-EM map of the 70S ribosome and tmRNA complex

1zc8, resolution 13.00Å

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